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DOI :
10.3791/63745-v
•
7:11 min
August 25th, 2022
Chapters
0:04
Introduction
1:25
Making Organoid Molds
2:22
Preparing Single‐Cell Suspension from Glioblastoma (GBM) Adherent Culture
3:22
Making Organoids from a Single‐Cell Suspension
5:23
Results: Generation and Maintenance of Human GBM Diversity Ex Vivo from Patient‐Derived Cell Cultures
6:10
Conclusion
여기에서는 1차 환자 검체 또는 환자 유래 세포 배양에서 교모세포종(GBM) 오가노이드를 생성하고 성숙할 때까지 유지하는 방법을 설명합니다. 이러한 GBM 오가노이드는 표현형적으로 다양한 세포 집단을 포함하고 생체 외 종양 미세 환경을 재현합니다.
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