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DOI :
10.3791/63779-v
•
8:14 min
December 9th, 2022
Chapters
0:05
Introduction
0:44
Raw Sample Preparation
1:56
Cell Lysing and Fractionation of Free Non-Protein Amino Acids (NPAAs)
3:36
Fractionation of the Protein-Bound Non-Protein Amino Acids (NPAAs)
6:11
Sample Filtering
6:35
Results: Extraction of NPAAs from Biological Matrices via TCA Protein Precipitation and Acid Hydrolysis
7:15
Conclusion
본 프로토콜은 액체 크로마토 그래피-탠덤 질량 분석법을 사용하여 분석하기 전에 트리클로로 아세트산 (TCA) 단백질 침전 및 산 가수 분해 를 통해 생물학적 매트릭스에서 비 단백질 아미노산을 추출하는 것을 설명합니다.
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