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DOI :
10.3791/63799-v
June 27th, 2022
Chapters
0:04
Introduction
1:06
Embryo Incubation and Preparation of Culture Dishes
1:49
Isolation of Chick Embryos
3:01
Dissecting Neural Folds
4:22
Plating Neural Folds
5:20
Fixing and Staining of Cultured Migratory Neural Crest Cells for Morphological Analysis
7:08
Results: Assessment of Cultured Migratory Neural Crest Cells
8:39
Conclusion
この汎用性の高いプロトコルは、ニワトリ胚からの脳神経襞の切除による遊走性神経堤細胞(NCC)の単離について説明しています。プレーティングとインキュベーションを行うと、遊走性NCCが神経襞の外植片から出現し、単純化された2D環境での細胞形態と遊走の評価を可能にします。
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