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JoVE Journal

Bioengineering

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MEDIA_ELEMENT_ERROR: Format error

Isogenic Kidney Glomerulus Chip Engineered from Human Induced Pluripotent Stem Cells

2.7K Views

10:23 min

November 4th, 2022

November 4th, 2022

2,738 Views

0:04

Introduction

0:59

Preparation of Microfluidic Organ Chip Devices

1:31

Seeding of viECs and Intermediate Mesoderm Cells into the Microfluidic Device

7:15

Podocyte Induction and Chip Maintenance

8:03

Results: Differentiation of viECs and Podocytes on Organ-On-A-Chip Device

9:35

Conclusion

Transcript

The isogenic glomerulus chip will enable patient specific disease modeling and nephrotoxicity testing and advanced precision medicine applications. Glomerular epithelium and endothelium are derived from a single population of human-induced pluripo

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Presented here is a protocol to engineer a personalized organ-on-a-chip system that recapitulates the structure and function of the kidney glomerular filtration barrier by integrating genetically matched epithelial and vascular endothelial cells differentiated from human induced pluripotent stem cells. This bioengineered system can advance kidney precision medicine and related applications.

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