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DOI :
10.3791/63891-v
May 5th, 2022
Chapters
0:04
Introduction
0:40
Polydimethylsiloxane (PDMS) Chamber Assembly
1:45
Connect Microfluidic Device
3:11
Configuring the Setup with Standard Flow Rates and Changing Solution
4:21
Basic Single Filament Experiment: Adenosine Diphosphate (ADP)-Actin Barbed End Depolymerization
6:45
Results: Polymerization of Actin Filaments from Spectrin-Actin Seeds when Exposed to Different Actin Binding Proteins
7:24
Conclusion
我々は、蛍光顕微鏡法と組み合わせて、個々のアクチンフィラメントをリアルタイムで正確に監視しながら、異なるタンパク質溶液に順次曝露することを可能にする、単純なアクチンフィラメントマイクロ流体アッセイのプロトコルを提示する。
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