Name: functional assessment of the donor heart during ex situ perfusion: insights from pressure-volume loops and surface echocardiography
Uploaded: 2022-10-11T13:20:00
Description: Watch this Scientific Journal Video about Functional Assessment of the Donor Heart During Ex Situ Perfusion: Insights from Pressure-Volume Loops and Surface Echocardiography at JoVE.com

乔治·洛佩兹研究所， 利苏， 69380， 法国
克劳迪娅·拉塞尔达，通用电气医疗保健，法国布克

本协议已获得当地动物实验伦理委员会和动物福利机构委员会（APAFIS#30483-2021031811339219 v1，法国巴黎萨克雷大学动物伦理委员会）的批准。动物按照美国国立卫生研究院制定的实验动物护理和使用指南和国家医学研究学会制定的实验动物护理原则进行处理。
注意：外科手术是在严格的无菌下进行的，使用与人类相同的技术。实验程序包括大白仔猪（45-60公斤），并在全身麻醉?...

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Annual report 2018 Eurotransplant International Foundation Available from: <a target="_blank" href="https://www.eurotransplant.org/cms/mediaobject.php?file=ET_Jaarv">https://www.eurotransplant.org/cms/mediaobject.php?file=ET_Jaarv</a> (2018)</li><li>Guglin, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=How+to+increase+the+utilization+of+donor+hearts.">How to increase the utilization of donor hearts.</a> Heart Failure Reviews. 20 (1), 95-105 (2015).</li><li>Tuttle-Newhall, J. E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Organ+donation+and+utilization+in+the+United+States:+1998-2007.">Organ donation and utilization in the United States: 1998-2007.</a> American Journal of Transplantation. 9 (4), 879-893 (2009).</li><li>Dronavalli, V. B., Banner, N. R., Bonser, R. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Assessment+of+the+potential+heart+donor.">Assessment of the potential heart donor.</a> Journal of the American College of Cardiology. 56 (5), 352-361 (2010).</li><li>Reich, H. J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Effects+of+older+donor+age+and+cold+ischemic+time+on+long-term+outcomes+of+heart+transplantation.">Effects of older donor age and cold ischemic time on long-term outcomes of heart transplantation.</a> Texas Heart Institute Journal. 45, 17-22 (2018).</li><li>Dhital, K. K., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Adult+heart+transplantation+with+distant+procurement+and+ex-vivo+preservation+of+donor+hearts+after+circulatory+death:+a+case+series.">Adult heart transplantation with distant procurement and ex-vivo preservation of donor hearts after circulatory death: a case series.</a> The Lancet. 385 (9987), 2585-2591 (2015).</li><li>Garry, B. P., Bivens, H. E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+Seldinger+technique.">The Seldinger technique.</a> Journal of Cardiothoracic Anesthesia. 2 (3), 403 (1988).</li><li>Lang, R. M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Recommendations+for+cardiac+chamber+quantification+by+echocardiography+in+adults:+an+update+from+the+American+Society+of+Echocardiography+and+the+European+Association+of+Cardiovascular+Imaging.">Recommendations for cardiac chamber quantification by echocardiography in adults: an update from the American Society of Echocardiography and the European Association of Cardiovascular Imaging.</a> Journal of the American Society of Echocardiography. 28 (1), 1-39 (2015).</li><li>White, C. W., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Assessment+of+donor+heart+viability+during+ex+situ+heart+perfusion.">Assessment of donor heart viability during ex situ heart perfusion.</a> Canadian Journal of Physiology and Pharmacology. 93 (10), 893-901 (2015).</li><li>Hatami, S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Myocardial+functional+decline+during+prolonged+ex+situ+heart+perfusion.">Myocardial functional decline during prolonged ex situ heart perfusion.</a> Annals of Thoracic Surgery. 108 (2), 499-507 (2021).</li><li>Hatami, S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+position+of+the+heart+during+normothermic+ex+situ+heart+perfusion+is+an+important+factor+in+preservation+and+recovery+of+myocardial+function.">The position of the heart during normothermic ex situ heart perfusion is an important factor in preservation and recovery of myocardial function.</a> American Society of Artificial Internal Organs Journal. 67 (11), 1222-1231 (2021).</li><li>Hatami, S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Normothermic+ex+situ+heart+perfusion+in+working+mode:+assessment+of+cardiac+function+and+metabolism.">Normothermic ex situ heart perfusion in working mode: assessment of cardiac function and metabolism.</a> Journal of Visualized Experiments. (143), e58430 (2019).</li><li>Tchouta, L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Twenty-four-hour+normothermic+perfusion+of+isolated+ex+situ+hearts+using+plasma+exchange.">Twenty-four-hour normothermic perfusion of isolated ex situ hearts using plasma exchange.</a> Journal of Thoracic and Cardiovascular Surgery. 164 (1), 128-138 (2020).</li><li>Ribeiro, R., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Comparing+donor+heart+assessment+strategies+during+ex+situ+heart+perfusion+to+better+estimate+posttransplant+cardiac+function.">Comparing donor heart assessment strategies during ex situ heart perfusion to better estimate posttransplant cardiac function.</a> Transplantation. 104 (9), 1890-1898 (2020).</li><li>Guihaire, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Are+pressure-volume+loops+relevant+for+hemodynamic+assessment+during+ex+vivo+heart+perfusion.">Are pressure-volume loops relevant for hemodynamic assessment during ex vivo heart perfusion.</a> Journal of Heart and Lung Transplantation. 39 (10), 1165-1166 (2020).</li><li>Hamed, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Serum+lactate+is+a+highly+sensitive+and+specific+predictor+of+post+cardiac+transplant+outcomes+using+the+Organ+Care+System.">Serum lactate is a highly sensitive and specific predictor of post cardiac transplant outcomes using the Organ Care System.</a> Journal of Heart and Lung Transplantation. 28 (2), 71 (2009).</li></ol>

NESP协议中需要考虑一些关键步骤。对心脏进行 原位 初步评估仍然很重要，特别是考虑到主动脉瓣不应出现明显的主动脉瓣反流（2 级或以上）;否则，由于冠状动脉灌注受损和心肌缺血，在朗根多夫期间心脏的复苏将受到影响。朗根多夫灌注后启动WM是一项具有挑战性的操作，至少需要两个人来调节预负荷储液器的填充、泵流量、左心房压力和主动脉流出管。一旦达到乳酸的代谢有效心肌?...

心脏移植是晚期心力衰竭的金标准治疗，但受到当前器官短缺的限制1。越来越多的供体心脏具有扩展标准（年龄>45岁、心血管危险因素、长期低流量、继发于儿茶酚胺能危象的急性左心室功能障碍）被分配，原发性移植失败的风险增加2。此外，在控制循环死亡（DCD）后捐赠的心脏可能会出现继发于长期温缺血的心肌损伤3。因此，有必要在移植前对这些供体心脏进行更好的评估，特别是评估它们是否有资格进行心脏移植4，5。 
常母异 位 灌注（NESP）使用温氧血液保存跳动的心脏。NESP的唯一市售设备将心脏保持在非工作状态（朗根道夫模式）。这种方法最初用于将移植物的保存扩大到冷缺血的关键4小时期之后6。该技术的另一个主要优点是根据灌注液中的乳酸浓度提供心肌活力的连续评估6。然而，迄今为止，这种生化评估从未与移植后的结果相关。同样，NESP的朗根多夫模式不允许在移植前对心脏进行血流动力学和功能评估。一些作者报道了NESP期间心内导管插入术对预测移植后心肌恢复的潜在益处7。
本报告旨在提供一种可重复的方法来评估NESP期间供体的心脏性能。我们修改了电路以允许工作模式灌注，从而通过心外膜超声心动图获取无创功能变量。心肌功指数是一个与负荷无关的变量，使用压力-应变环路记录。我们研究了心肌功与心内导尿获得的血流动力学变量之间的关系。

<table border="1" fo:keep-together.within-page="1" fo:keep-with-next.within-page="always">
	<tbody>
		<tr>
			<td>3T Heater Cooler System</td>
			<td>Liva Nova, Ch&#226;tillon, France</td>
			<td>IM-00727 A</td>
			<td>Extracorporeal Heater Cooler device</td>
		</tr>
		<tr>
			<td>4-0 polypropylene suture</td>
			<td>Peters, bobigny, France</td>
			<td>20S15B</td>
			<td>sutures</td>
		</tr>
		<tr>
			<td>5-0 polypropylene suture</td>
			<td>Peters, bobigny, France</td>
			<td>20S10B</td>
			<td>sutures</td>
		</tr>
		<tr>
			<td>Adenosine</td>
			<td>Efisciens BV, Rotterdam, Netherlands</td>
			<td>9088309</td>
			<td>Drugs for the ex-vivo perfusion</td>
		</tr>
		<tr>
			<td>Adrenaline</td>
			<td>Aguettant, Lyon, France</td>
			<td>600040</td>
			<td>Drugs for the ex-vivo perfusion</td>
		</tr>
		<tr>
			<td>Atracurium</td>
			<td>Pfizer Holding France, Paris, France</td>
			<td>582547</td>
			<td>Drugs for the induction of the anesthesia</td>
		</tr>
		<tr>
			<td>DeltaStream</td>
			<td>Fresenius Medical Care, L&#8217;Arbresle, France</td>
			<td>MEH2C4024</td>
			<td>Extracorporeal blood pump</td>
		</tr>
		<tr>
			<td>EKG epicardial electrodes</td>
			<td>Cardinal Health LLC, Waukegan, Illinois, USA</td>
			<td>31050522</td>
			<td>EKG detection electrodes</td>
		</tr>
		<tr>
			<td>External pacemaker</td>
			<td>Medtronic Inc. Minneapolis, Minneapolis, USA</td>
			<td>5392</td>
			<td>Pacemaker device</td>
		</tr>
		<tr>
			<td>Glucose 5%</td>
			<td>B.Braun Melsungen AG, Melsungen, Germany</td>
			<td>3400891780017</td>
			<td>Drugs for the priming solution</td>
		</tr>
		<tr>
			<td>Heart Perfusion Set, Organ Care System</td>
			<td>Transmedics, Andover, MA, USA</td>
			<td>Ref#1200</td>
			<td>Normothermic ex-vivo heart perfusion device</td>
		</tr>
		<tr>
			<td>Intellivue MX550</td>
			<td>Philips Healthcare, Suresnes, France</td>
			<td>NA</td>
			<td>Permanent monitoring system</td>
		</tr>
		<tr>
			<td>Istat 1</td>
			<td>Abbott, Chicago, Ill, USA</td>
			<td>714336-03O</td>
			<td>Blood Analyzer machine</td>
		</tr>
		<tr>
			<td>Labchart</td>
			<td>AD Instruments Ltd, Paris, France</td>
			<td>LabChart&#160;v8.1.21</td>
			<td>Pressure Volume loops aquisition software</td>
		</tr>
		<tr>
			<td>Magnesium</td>
			<td>Aguettant, Lyon, France</td>
			<td>564 780-6</td>
			<td>Drugs for the cardioplegia</td>
		</tr>
		<tr>
			<td>Magnesium Sulfate</td>
			<td>Aguettant, Lyon, France</td>
			<td>600111</td>
			<td>Drugs for the cardioplegia</td>
		</tr>
		<tr>
			<td>Mannitol 20%</td>
			<td>Macopharma, Mouvoux, France</td>
			<td>3400891694567.00</td>
			<td>Drugs for the cardioplegia</td>
		</tr>
		<tr>
			<td>Methylprednisolone</td>
			<td>Mylan S.A.S, Saint Priest, France</td>
			<td>400005623</td>
			<td>Drugs for the priming solution</td>
		</tr>
		<tr>
			<td>Millar Conductance Catheter</td>
			<td>AD Instruments Ltd, Paris, France</td>
			<td>Ventri-Cath 507</td>
			<td>Pressure Volume loops conductance catheter</td>
		</tr>
		<tr>
			<td>MWI software</td>
			<td>General Electric Healthcare, Chicago, Ill, USA</td>
			<td>NA</td>
			<td>software used for the Ultrasound echocardiographic machine</td>
		</tr>
		<tr>
			<td>Orotracheal probe</td>
			<td>Smiths medical ASD, Inc., Minneapolis, Minneapolis, USA</td>
			<td>100/199/070</td>
			<td>probe for the intubation during anesthesia</td>
		</tr>
		<tr>
			<td>Potassium chloride 10%</td>
			<td>B.Braun Melsungen AG, Melsungen, Germany</td>
			<td>3400892691527.00</td>
			<td>Drugs for the cardioplegia</td>
		</tr>
		<tr>
			<td>Propofol</td>
			<td>Zoetis France, Malakoff, France</td>
			<td>8083511</td>
			<td>Drugs for the induction of the anesthesia</td>
		</tr>
		<tr>
			<td>Quadrox-I small Adult Oxygenator</td>
			<td>Getinge, G&#246;teborg, Sweden</td>
			<td>BE-HMO 50000</td>
			<td>Extracorporeal blood oxygenator</td>
		</tr>
		<tr>
			<td>Ringer solution</td>
			<td>B.Braun Melsungen AG, Melsungen, Germany</td>
			<td>DKE2323</td>
			<td>Drugs for the cardioplegia</td>
		</tr>
		<tr>
			<td>Sodium Bicarbonate</td>
			<td>Laboratoire Renaudin, itxassou, France</td>
			<td>3701447</td>
			<td>Drugs for the cardioplegia</td>
		</tr>
		<tr>
			<td>Sodium chloride</td>
			<td>Aguettant, Lyon, France</td>
			<td>606726</td>
			<td>Drugs for the priming solution</td>
		</tr>
		<tr>
			<td>Swan Ganz Catheter</td>
			<td>Merit Medical, south jordan, utah, USA</td>
			<td>5041856</td>
			<td>Right pressure and cardiac output probe</td>
		</tr>
		<tr>
			<td>Tiletamine</td>
			<td>Virbac France, Carros, France</td>
			<td>3597132126021.00</td>
			<td>Drugs for the induction of the anesthesia</td>
		</tr>
		<tr>
			<td>Transesophagus probe (3&#8211;8 MHz 6VT)</td>
			<td>General Electric Healthcare, Chicago, Ill, USA</td>
			<td>NA</td>
			<td>Ultrasound echocardiographic transesophagus probe</td>
		</tr>
		<tr>
			<td>Vivid E95 ultraSound Machine</td>
			<td>General Electric Healthcare, Chicago, Ill, USA</td>
			<td>NA</td>
			<td>Ultrasound echocardiographic machine</td>
		</tr>
		<tr>
			<td>Xyloca&#239;ne 2%</td>
			<td>Aspen, Reuil-malmaison, France</td>
			<td>600550</td>
			<td>Drugs for the cardioplegia</td>
		</tr>
		<tr>
			<td>Zolazepam</td>
			<td>Virbac France, Carros, France</td>
			<td>3597132126021.00</td>
			<td>Drugs for the induction of the anesthesia</td>
		</tr>
	</tbody>
</table>

functional assessment of the donor heart during ex situ perfusion: insights from pressure-volume loops and surface echocardiography

心脏移植仍然是晚期心力衰竭的金标准治疗。然而，目前的关键器官短缺导致越来越多的捐赠者心脏被分配，并具有扩展标准。这些边缘移植物与原发性移植失败的高风险相关，移植前的 异位 灌注可能有益。该技术允许使用温氧血液灌注和连续代谢监测来扩展器官保存。目前唯一可用于临床实践的NESP设备在卸载的非工作状态下灌注器官，这不允许对跳动的心脏进行功能评估。因此，我们开发了一种在工作模式下的NESP原始平台，可以调整左心室前负荷和后负荷。该方案应用于猪心。通过心内导尿术和表面超声心动图实现心脏的 离位 功能评估。除了对实验方案的描述外，我们在此报告了主要结果，以及与NESP期间获得压力 - 容量环和心肌功率相关的珍珠和陷阱。血流动力学发现与超声变量之间的相关性是主要兴趣，特别是对于移植前供体心脏的进一步康复。该协议旨在改善对供体心脏的评估，以增加供体库并降低原发性移植失败的发生率。

我们在此描述了一种处于单心室工作状态的NESP方案，使用通常在临床实践中采用的改良心脏灌注模块，用于移植前供体心脏的Langendorff灌注。这种使用当前定制模块的NESP仔猪模型是在2019年开发的。电路的修改很小，因为大部分灌注回路被重新用于实验。模块的盖子提供了一层柔性和防水膜，在运输过程中保护心脏。它还允许在无菌环境中进行表面超声心动图检查。临床实践中混合血液和灌注液?...

Watch this Scientific Journal Video about Functional Assessment of the Donor Heart During Ex Situ Perfusion: Insights from Pressure-Volume Loops and Surface Echocardiography at JoVE.com

Functional Assessment of the Donor Heart During Ex Situ Perfusion: Insights from Pressure-Volume Loops and Surface Echocardiography

缺乏可靠的无创方法，用于在正常变温非 原位 心脏灌注（NESP）期间对供体心脏进行功能评估。我们在此描述了使用心外膜超声心动图和电导导管方法对心肌性能进行 异位 评估的方案。

非原位灌注期间供体心脏的功能评估：来自压力-容量环和表面超声心动图的见解

Heart transplantation remains the gold standard treatment for advanced heart failure. However, the current critical organ shortage has resulted in the ...

孤立体外位灌注期间心脏的血流动力学评估描述不佳。该协议提供了一种在工作模式下评估非原位心脏性能的方法。我们的方法将有创PV环技术与心外膜超声心动图技术进行了比较，以在孤立的离位灌注期间对其心脏进行功能评估。这种方法需要团队在非原位心脏灌注方面的经验，以及接受过经食管超声心动图培训的心脏病专家的参与。心脏移植的一个主要问题是原发性移植失败的风险。我们使用心外膜超声心动图的方法在非原位灌注期间提供移植前的心脏评估。这种非侵入性方法可以在移植前的异位心脏灌注期间应用。首先在麻醉动物的右心房和升主动脉上放置四条零聚丙烯缝合线，以用止血带固定插管。输注肝素并仔细解剖主动脉根部。然后在右心房插入双期静脉插管用于采血，在升主动脉插入单腔插管用于输注心脏停搏。分离上腔静脉和下腔静脉后，将静脉套管连接到含有10， 000单位普通肝素的采血袋上。将仔猪体置于特伦德伦贝格位置，以改善血液流入收集袋。进行心脏采集，保持肺干两厘米以及左心房后壁。将心脏置于四摄氏度的盐水溶液中，并将升主动脉与肺干分开。确认主动脉瓣和冠状动脉骨没有受伤。在升主动脉远端下方5毫米处插入四个承诺的四个零聚丙烯缝线，并将输液插管插入主动脉。拧紧主动脉周围的软管夹以固定套管。将引流插管插入肺干，并用三零聚丙烯运行缝合线固定。用五条零聚丙烯运行缝合线关闭下腔静脉和上腔静脉。用四零聚丙烯线缝合左心房后壁。将左通气插管插入左心房后壁，并在周围缠上止血带。将预装套管插入左心耳，并在周围缠绕止血带。将泵流量降低到每分钟 200 毫升。在给连接器放气后，将心脏连接到主动脉连接器。在室温下将主动脉压调节至 30 毫米汞柱。在复苏期间，进行平滑的心脏按摩，直到窦性心律恢复。在 15 到 25 分钟内以每分钟 50 毫升的步长缓慢增加泵流量，以达到 65 毫米汞柱的主动脉压。同时，将大量温度提高两到四摄氏度，达到37摄氏度。一旦达到所需的主动脉压和大量温度，如果需要，在五焦耳处进行电击，并重复直到窦性心律恢复。将心外膜电极固定在右心室后壁上，并连接到外部起搏器。然后将肺导管连接到冠状动脉血流线。打开预压管路，调节泵流量，确保预紧油箱稳定加注。打开主动脉后负荷管路并夹紧用于兰甘多夫灌注的回路的主线。后负荷水库将逐步填满。确保通过溢流管将溢流管排出，将溢流管带回回路的主储液罐。以每分钟0.04毫克的速度开始输注多巴酚丁胺。然后拆下通风口。用盐水溶液清洁七个法国尾纤电导导管，并将其连接到硬件接口。轻轻地将导管推入先前通过左心房顶部插入的引入器8法式鞘中，以与二尖瓣对齐。停止心脏的心外膜起搏，以避免干扰电导信号。当信号稳定时，以稳定状态记录数据。选择一系列 10 个连续循环并打开分析软件。要获得收缩末期和舒张末期压容积关系，请记录预负荷闭塞期间的信号。逐渐夹紧心房充血管，直到前负荷减轻有效。然后慢慢松开夹子。在心脏周围放置无菌窗帘后，将探头敷在左心房的上壁上，并手动旋转换能器，直到获得四腔视图。然后运行超声探头监视器以获得三个和两个腔室视图。为了评估MWI，需要获取4、3和2腔视图，并记录同时动脉压。使用这些视图和打开 MWI 软件评估全球纵向应变。使用循环采集在灌注回路上的外部传感器检测到的侵入性血压。手动通知软件主动脉瓣和二尖瓣的确切打开和关闭时间。在自定义设置中执行工作模式灌注期间的超声心动图评估。所有三个左心室视图都是在所有实验的任何时间点获得的。平均左心室射血分数整体纵向应变和M W I分别约为40.8%-8.0%和65毫米汞柱。在工作模式灌注期间，通过电导导管或表面超声心动图评估血流动力学参数。在所有实验中，在工作模式下，随着时间的推移，观察到M W I显着降低。在心输出量和与收缩末期压容积关系相关的其他参数中观察到类似的下降。超声心动图探头必须位于左心房的顶部。必须正确执行至少三到六个心肌周期的稳定要求。对心肌组织和内皮功能的代谢评估将在非原位心脏灌注期间对心脏移植物进行补充评估，以优化灌注设置。

Research

JoVE Journal

Medicine

Functional Assessment of the Donor Heart During Ex Situ Perfusion: Insights from Pressure-Volume Loops and Surface Echocardiography

Myocardial Infarction and Functional Outcome Assessment in Pigs

心肌梗死和功能预后评估的猪

Introduction of newly discovered cardiovascular therapeutics into first-in-man trials depends on a strictly regulated ethical and legal roadmap. One ...

科研

医学

Technique of Subnormothermic Ex Vivo Liver Perfusion for the Storage, Assessment, and Repair of Marginal Liver Grafts

Technique of Subnormothermic Ex Vivo Liver Perfusion for the Storage, Assessment, and Repair of Marginal Liver Grafts

Subnormothermic的技术<em&gt;前体内</em&gt;肝脏灌注的存储，评估和修复边际移植肝

The success of liver transplantation has resulted in a dramatic organ shortage. In most transplant regions 20-30% of patients on the waiting list for ...

Method of Isolated Ex Vivo Lung Perfusion in a Rat Model: Lessons Learned from Developing a Rat EVLP Program

Method of Isolated Ex Vivo Lung Perfusion in a Rat Model: Lessons Learned from Developing a Rat EVLP Program

的隔离方法<em&gt;离体</em&gt;肺灌注的大鼠模型：教训制定鼠EVLP课程中学

The number of acceptable donor lungs available for lung transplantation is severely limited due to poor quality. Ex-Vivo Lung Perfusion (EVLP) has allowed ...

Measurement of the Pressure-volume Curve in Mouse Lungs

In recent decades the mouse has become the primary animal model of a variety of lung diseases. In models of emphysema or fibrosis, the essential ...

Cardiac Catheterization in Mice to Measure the Pressure Volume Relationship: Investigating the Bowditch Effect

心导​​管在小鼠测量压力体积的关系：调查的影响鲍迪奇

Animal models that mimic human cardiac disorders have been created to test potential therapeutic strategies. A key component to evaluating these ...

Ex Situ Normothermic Machine Perfusion of Donor Livers

Ex Situ Normothermic Machine Perfusion of Donor Livers

供肝易地常温灌注机

In contrast to conventional static cold preservation (0-4 &#176;C), ex situ machine perfusion may provide better preservation of donor livers. Continuous ...

Real-time Pressure-volume Analysis of Acute Myocardial Infarction in Mice

小鼠急性心肌梗死的实时压力容积分析

Acute myocardial infarction can lead to acute heart failure and cardiogenic shock. The evaluation of hemodynamics is critical for the evaluation of any ...

Normothermic Ex Situ Heart Perfusion in Working Mode: Assessment of Cardiac Function and Metabolism

工作模式下的心功能和代谢评估

The current standard method for organ preservation (cold storage, CS), exposes the heart to a period of cold ischemia that limits the safe preservation ...

Biventricular Assessment of Cardiac Function and Pressure-Volume Loops by Closed-Chest Catheterization in Mice

小鼠闭胸导管插入术对心功能和压力-容积环的双心室评估

Assessment of cardiac function is essential to conduct cardiovascular and pulmonary-vascular preclinical research. Pressure-volume loops (PV loops) ...