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DOI :
10.3791/63999-v
•
9:00 min
June 10th, 2022
Chapters
0:04
Introduction
0:51
Tissue Dissection and Cleaning
4:39
Digestion of Isolated Arteries for Isolation of Endothelial Cells
6:01
Staining of Endothelial Cells Prior to Flow Cytometry
7:06
Results: Identification of Endothelial Cells Following Arterial Tissue Digestion
8:15
Conclusion
该协议详细介绍了一种解剖小鼠脂肪库以及分离和消化各自动脉以释放然后鉴定内皮细胞群的方法。用于下游应用的新鲜分离细胞将促进对血管细胞生物学和血管功能障碍机制的理解。
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