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DOI :
10.3791/63999-v
•
9:00 min
June 10th, 2022
Chapters
0:04
Introduction
0:51
Tissue Dissection and Cleaning
4:39
Digestion of Isolated Arteries for Isolation of Endothelial Cells
6:01
Staining of Endothelial Cells Prior to Flow Cytometry
7:06
Results: Identification of Endothelial Cells Following Arterial Tissue Digestion
8:15
Conclusion
このプロトコルは、マウス脂肪デポーの解剖および内皮細胞集団を遊離させてから同定するためのそれぞれの動脈の単離および消化のための方法を詳述する。ダウンストリームアプリケーションで使用される新たに単離された細胞は、血管細胞生物学と血管機能障害のメカニズムの理解を促進します。
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