Name: assay for adhesion and agar invasion in s. cerevisiae
Uploaded: 2006-11-08T00:00:00
Description: Watch this Scientific Journal Video about Assay for Adhesion and Agar Invasion in S. cerevisiae at JoVE.com

我们要感谢他们的真知灼见丽莎Schneper和卡特琳Duevel发展这个实验。

<ol><li>将200ul合成介质板所需的饥饿条件（SC SC 2％和0.2％的葡萄糖与葡萄糖，例如）的兴趣不断增长的文化，如果文化的密度过彼此不同，调整细胞计数每200ul文化所以每下降大约相同数量的细胞。 </li><li>请一定要保持板块的下跌是文化的记录。 </li><li>保持板盖半掩，留在室温或在30 ° C，直到滴干。 </li><li>封口膜和保鲜膜（可选）的密封板和留在30℃为3-7天。 </li><li>文件（通过扫描，数字图片等）平板上的...

<ol>
	<li>Costerton, J. W., Lewandowski, Z., Caldwell, D. E., Korber, D. R., Lappin-Scott, H. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Microbial+biofilms.">Microbial biofilms.</a> Annu Rev Microbiol. 49, 711-745 (1995).</li><li>Elortondo, F. J. P., Salmeron, J., Albisu, M., Casas, C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Biofilms+in+the+food+industry.">Biofilms in the food industry.</a> Food Science and Technology International. 5, 25-30 (1999).</li><li>Keinanen, M. M., Martikainen, P. J., Kontro, M. 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酵母细胞显示各种分化的模式，根据营养可用性和环境条件，包括饥饿和压力的条件下，根据各种营养素强调的细丝，和絮凝孢子形成。各种酵母菌，包括酵母和白色念珠菌，也可以发现在不同的微生物形成的生物膜。虽然有一些丝和入侵行为的相关性，目前尚不清楚究竟如何丝可能会导致表面和组织的入侵和定植。酵母可以肯定会发现在营养和丝状的形式在自然界中生物膜，以及威胁人类健康的地方，如导管?...

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<td>Moticam 350</td>
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<td>Motic</td>
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assay for adhesion and agar invasion in s. cerevisiae

酵母菌是在自然生物膜发现，许多微生物殖民表面。在人工环境，如人造物体表面，生物膜可以减少工业生产力，破坏结构，威胁着人类的生活。 1-3另一方面，利用生物膜的力量可以帮助清洁环境，并产生可持续的能源。 4-8酿酒酵母拓殖表面，并参与复杂的生物膜的能力主要是通过各种信号转导通路，并在这个有机体的环境因素引发的分化程序的重新发现被忽略，直到。 9，10使用酿酒酵母作为一种模式生物，理解的互动和衔接的信号转导通路的Ras - PKA，Kss1 MAPK和Hog1渗透压的途径，如迅速放置在酿酒酵母中生物膜的交界处，持续关注生物学和信号转导研究。 11-20为此，酵母细胞分化成长，胶粘剂，假菌丝细丝成为一个方便读数在各种环境变化的信号转导通路的激活。然而，丝是一个复杂的表型，这使得它的化验就好像它是一个简单的表型误导的集合。在过去的十年中，成功地采用了一些分析，从细菌生物膜研究酵母的研究，如垫形成实验测量软琼脂和结晶紫染色法，定量测量细胞表面坚持殖民地蔓延，。 12日，21然而，有一些发达国家的检测，定性评估的粘合剂和侵入表型酵母琼脂混乱。在这里，我们提出了一个简单和可靠的方法评估酵母菌株的粘合剂和侵入性的质量易于理解的步骤，以隔离入侵评估附着力评估。我们从以往的研究，10，16中采用的方法，包括生长的细胞在液体介质和电镀差大点，然后用清水洗净，以评估附着力和琼脂表面的细胞完全擦掉，以评估入侵到生长的营养条件琼脂。我们消除裸奔到琼脂细胞的需要，从而影响到琼脂细胞的侵袭。在一般情况下，我们观察到侵入单倍体菌株琼脂胶，但不是所有的粘合剂株可侵入琼脂培养基。我们的方法可以用来结合其他实验中，要认真剖析酵母的信号转导，分化，群体感应，和生物膜形成的分化步骤和要求。

Watch this Scientific Journal Video about Assay for Adhesion and Agar Invasion in S. cerevisiae at JoVE.com

Assay for Adhesion and Agar Invasion in S. cerevisiae

我们描述了酵母的附着力和琼脂作为衡量侵入和假菌丝分化入侵的定性分析。这个简单的实验，可用于评估各种突变体的侵入的表型，以及环境因素的影响，对酵母分化的信号通路。

化验粘附和酿酒酵母琼脂入侵

Yeasts are found in natural biofilms, where many microorganisms colonize surfaces. In artificial environments, such as surfaces of man-made objects, ...

Research

JoVE Journal

Biology

A high-throughput method to globally study the organelle morphology in S. cerevisiae

一种高通量的方法，在全球范围内研究在酿酒酵母中的细胞器形态

High-throughput methods to examine protein localization or organelle morphology is an effective tool for studying protein interactions and can help ...

科研

生物学

Identification of protein complexes with quantitative proteomics in S. cerevisiae

鉴定与定量蛋白质组学在酿酒酵母中的蛋白质复合物

Lipids are the building blocks of cellular membranes that function as barriers and in compartmentalization of cellular processes, and recently, as ...

An In vitro FluoroBlok Tumor Invasion Assay

An In vitro FluoroBlok Tumor Invasion Assay

一个<em在体外 FluoroBlok肿瘤侵袭实验

The hallmark of metastatic cells is their ability to invade through the basement membrane and migrate to other parts of the body. Cells must be able to ...

Agar-Block Microcosms for Controlled Plant Tissue Decomposition by Aerobic Fungi

为控制植物组织分解好氧菌的琼脂块缩影

The two principal methods for studying fungal biodegradation of lignocellulosic plant tissues were developed for wood preservative testing (soil-block; ...

A Fluorescence Microscopy Assay for Monitoring Mitophagy in the Yeast Saccharomyces cerevisiae

A Fluorescence Microscopy Assay for Monitoring Mitophagy in the Yeast Saccharomyces cerevisiae

在酵母用于监测Mitophagy荧光显微镜检测酿酒酵母

Autophagy is important for turnover of cellular components under a range of different conditions. It serves an essential homeostatic function as well as a ...

Studying Age-dependent Genomic Instability using the S. cerevisiae Chronological Lifespan Model

Studying Age-dependent Genomic Instability using the S. cerevisiae Chronological Lifespan Model

研究年龄相关的基因组不稳定性使用 S。酵母年代的寿命模型

Studies using the Saccharomyces cerevisiae aging model have uncovered life span regulatory pathways that are partially conserved in higher eukaryotes1-2. ...

Isolation of Primary Mouse Trophoblast Cells and Trophoblast Invasion Assay

分离小鼠原代滋养细胞及滋养细胞侵袭实验

The placenta is responsible for the transport of nutrients, gasses and growth factors to the fetus, as well as the elimination of wastes.  
Thus, defects ...

Flow Cytometry-based Purification of S. cerevisiae Zygotes

Flow Cytometry-based Purification of S. cerevisiae Zygotes

流式细胞仪分离纯化<em&gt; S.酵母</em&gt;合子

Zygotes are essential intermediates between haploid and diploid states in the life cycle of many organisms, including yeast (Figure 1) 1. S. cerevisiae ...

Visualization and Analysis of mRNA Molecules Using Fluorescence In Situ Hybridization in Saccharomyces cerevisiae

Visualization and Analysis of mRNA Molecules Using Fluorescence In Situ Hybridization in Saccharomyces cerevisiae

可视化和分析的mRNA分子，使用荧光<em&gt;在原位</em&gt;杂交<em&gt;酿酒酵母（Saccharomyces cerevisiae）</em

The Fluorescence in situ Hybridization (FISH) method allows one to detect nucleic acids in the native cellular environment. Here we provide a protocol for ...

Coupled Assays for Monitoring Protein Refolding in Saccharomyces cerevisiae

Coupled Assays for Monitoring Protein Refolding in Saccharomyces cerevisiae

耦合检测监控蛋白复性<em&gt;酿酒酵母（Saccharomyces cerevisiae）</em

Proteostasis, defined as the combined processes of protein folding/biogenesis, refolding/repair, and degradation, is a delicate cellular balance that must ...