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DOI :
10.3791/64085-v
•
8:13 min
September 16th, 2022
Chapters
0:05
Introduction
1:16
Animal Preparation
2:39
Heart Tissue Processing and Embedding
3:47
Tissue Sectioning Using an Ultramicrotome and Ultrathin Heart Section Staining
6:19
Transmission Electron Microscopy Imaging
6:48
Results: Visualization of Sigmar1 Using Quantum Dots
7:22
Conclusion
본 프로토콜은 양자점을 사용하여 심장 조직 절편에서 단백질을 면역표지하는 방법을 기술한다. 이 기술은 미세 구조 수준에서 모든 단백질의 세포 내 국소화 및 발현을 시각화하는 데 유용한 도구를 제공합니다.
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