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DOI :
10.3791/64103-v
•
8:12 min
August 23rd, 2022
Chapters
0:04
Introduciton
0:43
Preparation of 2% Agarose‐PBS Gel and Clearing Solution
1:39
Spheroid Preparation and Staining
4:04
Spheroid Mounting and Imaging
5:46
Results: Semi-High‐Throughput Imaging of 3D Tumor Spheroids
7:36
Conclusion
종양 구상체는 종양 세포-미세환경 상호작용 및 치료 반응을 평가하기 위해 점점 더 활용되고 있다. 본 프로토콜은 신속한 광학 클리어링을 사용하여 3D 종양 구상체의 반고처리량 이미징을 위한 견고하지만 간단한 방법을 기술한다.
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