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DOI :
10.3791/64135-v
September 23rd, 2022
Chapters
0:05
Introduction
1:04
Differential Centrifugation for Isolation of Exosomes and Microvesicles
2:25
Detection of Particle Numbers and Size Distribution of Extracellular Vesicles
3:29
Labeling of Extracellular Vesicles Derived from Mesenchymal Stem Cells
4:50
Systemic Injection of Extracellular Vesicles Derived from Mesenchymal Stem Cells
5:24
Results: Characterization of Extracellular Vesicles Derived from Mesenchymal Stem Cells
6:19
Conclusion
本プロトコルは、培養ヒト間葉系幹細胞から代表的なEV(エキソソームおよびマイクロベシクル)を単離および特性評価するための差動遠心分離について説明しています。これらのEVのさらなる用途についても、本稿で解説しています。
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