Induction of Senescence by Chemotherapy Drugs in Cultured Cancer Cells
3:29
DDAO-Galactoside Staining of Senescence-Associated Beta-Galactosidase (SA-β-Gal) in Cell or Tumor Samples
4:56
Flow Cytometer Setup and Data Acquisition
6:27
Flow Cytometry Data Analysis
9:01
Results: Screening Tumors and Chemotherapy Agents for the Induction of Senescence
13:06
Conclusion
Transcript
Our multi-parameter flow cytometry protocol enables rapid and improved detection of therapy-induced senescence in tumor cells, which is a condition of ongoing study in cancer biology and therapy. Our flow cytometry senescence assay is more rapid t
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A protocol for fluorescent, flow cytometric quantification of senescent cancer cells induced by chemotherapy drugs in cell culture or in murine tumor models is presented. Optional procedures include co-immunostaining, sample fixation to facilitate large batch or time point analysis, and the enrichment of viable senescent cells by flow cytometric sorting.