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2.7K Views
•
07:32 min
May 26th, 2023
DOI :
10.3791/64310-v
Chapters
0:05
Introduction
0:29
Aseptic Collection of Mouse Fecal Samples
1:21
Fecal Matter Transplantation (FMT)
2:06
Systolic Blood Pressure Measurements
2:47
Assessment of FMT to Cardiovascular Changes and Making Gut Intestine Swiss-Rolls
5:46
Results: Effects of High and Low-Salt Diet, Fecal Microbiota Transplantation from High-Salt Fed Mice, and Immunohistochemistry on the Ileum
7:07
Conclusion
Transcript
在这里,我们描述了一种协议,以指导其他人调查疾病中生态失调的机制,从收集粪便样本到使用瑞士卷法研究肠道变化。首先,用70%乙醇喷洒安乐死小鼠的胸部和侧面,并小心地打开皮肤和腹膜腔以暴露胃肠道。隔离盲肠,并使用无菌手术剪刀将其切成两半。
短暂暴露盲肠,从回肠近端切开 0.5 厘米,在与结肠的连接处远切 0.5 厘米。将分离的盲肠转移到无菌培养皿上。使用无菌刮刀将盲肠内容物转移到无菌管中,并将等分试样储存在 80 摄氏度的冰箱中。
将新鲜或先前冷冻的粪便颗粒以 1 至
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Summary
这里的目标是概述一个方案,以研究心血管疾病中生态失调的机制。本文讨论了如何无菌收集和移植鼠粪便样本,分离肠道,并使用“Swiss-roll”方法,然后使用免疫染色技术来询问胃肠道的变化。
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