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DOI :
10.3791/64365-v
•
9:23 min
March 10th, 2023
Chapters
0:04
Introduction
0:28
Cardiac Spheroid Generation
2:40
Cryopreservation of Cardiac Spheroids (CSs)
3:58
Thawing of Cardiac Spheroids
4:58
Assessment of Intracellular Ca2+ Transients
6:18
Results: Generation and Cryopreservation of Cardiac Spheroids from hiPSC‐CMs
8:34
Conclusion
这里介绍的是一组方案,用于从以高通量、多维形式培养的人诱导多能干细胞衍生心肌细胞中生成和冷冻保存心脏球体 (CS)。该三维模型可作为疾病建模、高通量筛选的强大平台,并在冷冻保存后保持其功能。
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