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1.6K Views
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10:06 min
September 2nd, 2022
DOI :
10.3791/64406-v
Chapters
0:05
Introduction
1:01
Preparation and Characterization of PLGA Nanoparticles
2:38
Preparation and Characterization of PNPE
3:24
bEVs Adhere to the PNPE
5:31
CLSM Analysis of Antigen Uptake
8:14
Results: Affinity of PNPE to bEVs and Uptake of Antigens
9:41
Conclusion
Transcript
我们开发了PLGA纳米颗粒稳定的皮克林乳液。Pickering乳液改善了细胞与抗原蛋白细胞的亲和力,诱导抗原的有效内化。纳米颗粒稳定的皮克林乳液易于制备和高效递送抗原。
此外,还使用一种方法来监测乳液对细胞的亲和力并详细说明内化。该协议为设计具有高细胞效率和高效抗原内化的新型制剂提供了见解,为开发高效疫苗提供了平台。首先,将100毫克聚乳酸-乙醇酸以4比1的比例加入10毫升丙酮和乙醇混合物中,作为油相。
将20毫升PVA水溶液置于通风橱下,并以每分钟400转的速度磁
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Summary
为了合理设计高效的佐剂,我们开发了聚乳酸-乙醇酸纳米颗粒稳定的皮克林乳液(PNPE)。PNPE具有独特的柔软性和疏水界面,可实现有效的细胞接触,并提供高含量的抗原负载,改善递送系统与抗原呈递细胞的细胞亲和力,并诱导抗原的有效内化。
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