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DOI :
10.3791/64414-v
April 21st, 2023
Chapters
0:07
Introduction
2:16
Preparation
3:15
Enucleation of the Mouse Eye Along with the Third Eyelid
4:03
Clearing the Extraneous Tissue
5:17
Dissection of the Enucleated Eye to Obtain Anterior and Posterior Eyecups
7:28
Isolation of Neural Retina and RPE Layers
8:16
Segmentation of Retinal and RPE Layers for Whole Mounts
9:38
Results
10:12
Conclusion
この論文は、げっ歯類の眼の微小解離のためのプロトコルを提示します。このプロセスには、刺激膜(すなわち、第3のまぶた)とともに眼球の除核が含まれます。その後、後眼カップと前眼カップが分離します。
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