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Microsampling in Targeted Mass Spectrometry-Based Protein Analysis of Low-Abundance Proteins

DOI :

10.3791/64473-v

10:21 min

January 13th, 2023

January 13th, 2023

1,761 Views

1Department of Pharmacy, University of Oslo

A protocol is presented for the determination of low-abundance biomarkers from dried serum samples exemplified with the biomarker progastrin-releasing peptide (ProGRP). Antibody-coated magnetic beads are used for the selective cleanup and enrichment of a proteotypic ProGRP peptide. The captured peptide is subsequently analyzed by liquid chromatography-tandem mass spectrometry.

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Microsampling

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