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Analyzing Protein Dynamics Using Hydrogen Exchange Mass Spectrometry

DOI :

10.3791/50839-v

November 29th, 2013

November 29th, 2013

17,820 Views

1Zentrum für Molekulare Biologie der Universität Heidelberg (ZMBH), University of Heidelberg

Protein conformation and dynamics are key to understanding the relationship between protein structure and function. Hydrogen exchange coupled with high-resolution mass spectrometry is a versatile method to study the conformational dynamics of proteins as well as characterizing protein-ligand and protein-protein interactions, including contact interfaces and allosteric effects.

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