Research
Education
Sign In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Please note that all translations are automatically generated. Click here for the English version.
DOI :
10.3791/64493-v
December 2nd, 2022
Chapters
0:04
Introduction
0:43
Preparation of Perfusion Equipment and Animal Surgery
2:51
Cannulation and Perfusion
5:03
Digestion
6:21
Preparation of the Liver
6:51
Hepatocyte Extraction and Isolation
8:11
Results: A Reliable and Straightforward Method for Isolating High Yield Normal and Steatotic Murine Hepatocytes
9:37
Conclusion
脂質を含んだ肝細胞は肝臓の再生に固有のものですが、通常は密度勾配遠心分離で失われます。ここでは、脂肪肝細胞を保持する最適化された細胞分離プロトコルを提示し、マウスの部分肝切除後に再生肝細胞の代表的な集団を生み出します。
Tags
-- Views
Related Videos
Privacy
Terms of Use
Policies
Contact Us
Recommend to library
JoVE NEWSLETTERS
JoVE Journal
Methods Collections
JoVE Encyclopedia of Experiments
Archive
JoVE Core
JoVE Business
JoVE Science Education
JoVE Lab Manual
Faculty Resource Center
Authors
Overview
Publishing Process
Editorial Board
Scope and Policies
Peer Review
FAQ
Submit
Librarians
Testimonials
Subscriptions
Access
Resources
Library Advisory Board
ABOUT JoVE
Leadership
Copyright © 2024 MyJoVE Corporation. All rights reserved