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DOI :
10.3791/64493-v
December 2nd, 2022
Chapters
0:04
Introduction
0:43
Preparation of Perfusion Equipment and Animal Surgery
2:51
Cannulation and Perfusion
5:03
Digestion
6:21
Preparation of the Liver
6:51
Hepatocyte Extraction and Isolation
8:11
Results: A Reliable and Straightforward Method for Isolating High Yield Normal and Steatotic Murine Hepatocytes
9:37
Conclusion
지질이 함유된 간세포는 간 재생에 내재되어 있지만 일반적으로 밀도 구배 원심분리 시 손실됩니다. 여기에서 우리는 지방성 간세포를 유지하는 최적화된 세포 분리 프로토콜을 제시하여 마우스에서 부분 간 절제술 후 재생 간세포의 대표적인 집단을 생성합니다.
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