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DOI :
10.3791/64572-v
•
9:16 min
February 3rd, 2023
Chapters
0:05
Introduction
0:53
Culturing Human Retinal Pigment Epithelial Cells (H-RPE)
2:03
Assay Preparation: The Day Before Running the Assay
2:43
Real-Time Mito Stress Test Using the Extracellular Flux Analyzer
7:26
Results: Oxygen Consumption Rate (OCR) and Extracellular Acidification Rate (ECAR)
8:42
Conclusion
ヒト網膜色素上皮細胞(H-RPE)の代謝状態は、その健康と機能を反映しています。ここでは、高解像度の呼吸測定を使用してH-RPEのリアルタイムの代謝フラックスを調べるための最適化されたプロトコルを紹介します。
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