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DOI :
10.3791/64572-v
•
9:16 min
February 3rd, 2023
Chapters
0:05
Introduction
0:53
Culturing Human Retinal Pigment Epithelial Cells (H-RPE)
2:03
Assay Preparation: The Day Before Running the Assay
2:43
Real-Time Mito Stress Test Using the Extracellular Flux Analyzer
7:26
Results: Oxygen Consumption Rate (OCR) and Extracellular Acidification Rate (ECAR)
8:42
Conclusion
인간 망막 색소 상피 세포(H-RPE)의 대사 상태는 건강과 기능을 반영합니다. 여기에 제시된 것은 고해상도 호흡 측정을 사용하여 H-RPE의 실시간 대사 플럭스를 검사하기 위한 최적화된 프로토콜입니다.
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