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DOI :
10.3791/64586-v
•
8:20 min
May 12th, 2023
Chapters
0:04
Introduction
0:55
Microfilament Preparation
1:56
Embryoid Body Formation
3:42
Neuroepithelial Induction
4:21
Organoid Embedding and Differentiation
6:39
Results: Characterization of the Organoids
7:40
Conclusion
本プロトコルは、ヒト誘導多能性幹細胞(iPSC)由来の脳オルガノイドの再現性のある生成、維持、および老化のための段階的な手順を提供します。この方法により、脳オルガノイドを長期間培養・成熟させることができ、脳の老化や加齢に伴う病態形成に関わる過程のモデル化が容易になります。
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