Name: single-anastomosis duodeno-ileal bypass with sleeve gastrectomy model in mice
Uploaded: 2023-02-10T14:05:00
Description: Watch this Scientific Journal Video about Single-Anastomosis Duodeno-Ileal Bypass with Sleeve Gastrectomy Model in Mice at JoVE.com

我们感谢Ethicon（强生外科技术）慷慨地提供缝合线和手术夹。这项工作得到了NExT人才项目，南特大学，南特CHU的资助。

该协议已获得当地法国动物实验伦理委员会的批准（Comité d'éthique en expérimentation animale;参考CEEA-PdL n 06）。
1.术前准备
<ol><li>手术前3天在正常饮食中添加凝胶饮食食品。手术前6小时禁食小鼠。</li>
	<li>在带氧气（1 L / min）的专用腔室中用5%异氟醚（1L / min）诱导麻醉。皮下注射丁丙诺啡（0.1mg / kg），阿莫西林（15mg / kg），甲氧氯普胺（1mg / kg），美洛昔康（1mg / ...

<ol>
	<li>Flegal, K. M., Carroll, M. D., Kit, B. K., Ogden, C. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Prevalence+of+obesity+and+trends+in+the+distribution+of+body+mass+index+among+US+adults,+1999-2010.">Prevalence of obesity and trends in the distribution of body mass index among US adults, 1999-2010.</a> JAMA. 307 (5), 491-497 (2012).</li><li>Sj&#246;str&#246;m, L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Association+of+bariatric+surgery+with+long-term+remission+of+type+2+diabetes+and+with+microvascular+and+macrovascular+complications.">Association of bariatric surgery with long-term remission of type 2 diabetes and with microvascular and macrovascular complications.</a> JAMA. 311 (22), 2297-2304 (2014).</li><li>Dyson, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Hepatocellular+cancer:+the+impact+of+obesity,+type+2+diabetes+and+a+multidisciplinary+team.">Hepatocellular cancer: the impact of obesity, type 2 diabetes and a multidisciplinary team.</a> Journal of Hepatology. 60 (1), 110-117 (2014).</li><li>S&#225;nchez-Pernaute, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Proximal+duodenal-ileal+end-to-side+bypass+with+sleeve+gastrectomy:+proposed+technique.">Proximal duodenal-ileal end-to-side bypass with sleeve gastrectomy: proposed technique.</a> Obesity Surgery. 17 (12), 1614-1618 (2007).</li><li>Himpens, J., Verbrugghe, A., Cadi&#232;re, G. B., Everaerts, W., Greve, J. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Long-term+results+of+laparoscopic+Roux-en-Y+Gastric+bypass:+evaluation+after+9+years.">Long-term results of laparoscopic Roux-en-Y Gastric bypass: evaluation after 9 years.</a> Obesity Surgery. 22 (10), 1586-1593 (2012).</li><li>S&#225;nchez-Pernaute, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Long-term+results+of+single-anastomosis+duodeno-ileal+bypass+with+sleeve+gastrectomy+(SADI-S).">Long-term results of single-anastomosis duodeno-ileal bypass with sleeve gastrectomy (SADI-S).</a> Obesity Surgery. 32 (3), 682-689 (2022).</li><li>Shoar, S., Poliakin, L., Rubenstein, R., Saber, A. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Single+anastomosis+duodeno-ileal+switch+(SADIS):+A+systematic+review+of+efficacy+and+safety.">Single anastomosis duodeno-ileal switch (SADIS): A systematic review of efficacy and safety.</a> Obesity Surgery. 28 (1), 104-113 (2018).</li><li>Rao, R. S., Rao, V., Kini, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Animal+models+in+bariatric+surgery--a+review+of+the+surgical+techniques+and+postsurgical+physiology.">Animal models in bariatric surgery--a review of the surgical techniques and postsurgical physiology.</a> Obesity Surgery. 20 (9), 1293-1305 (2010).</li><li>Lutz, T. A., Bueter, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+use+of+rat+and+mouse+models+in+bariatric+surgery+experiments.">The use of rat and mouse models in bariatric surgery experiments.</a> Frontiers in Nutrition. 3, 25 (2016).</li><li>Baud, G., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Bile+diversion+in+Roux-en-Y+Gastric+Bypass+modulates+sodium-dependent+glucose+intestinal+uptake.">Bile diversion in Roux-en-Y Gastric Bypass modulates sodium-dependent glucose intestinal uptake.</a> Cell Metabolism. 23 (3), 547-553 (2016).</li><li>Blanchard, C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Sleeve+gastrectomy+alters+intestinal+permeability+in+diet-induced+obese+mice.">Sleeve gastrectomy alters intestinal permeability in diet-induced obese mice.</a> Obesity Surgery. 27 (10), 2590-2598 (2017).</li><li>Ayer, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Techniques+of+sleeve+gastrectomy+and+modified+Roux-en-Y+Gastric+Bypass+in+mice.">Techniques of sleeve gastrectomy and modified Roux-en-Y Gastric Bypass in mice.</a> Journal of Visualized Experiments. (121), e54905 (2017).</li><li>Wang, T., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Comparison+of+diabetes+remission+and+micronutrient+deficiency+in+a+mildly+obese+diabetic+rat+model+undergoing+SADI-S+versus+RYGB.">Comparison of diabetes remission and micronutrient deficiency in a mildly obese diabetic rat model undergoing SADI-S versus RYGB.</a> Obesity Surgery. 29 (4), 1174-1184 (2019).</li><li>Wu, W., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Comparison+of+the+outcomes+of+single+anastomosis+duodeno-ileostomy+with+sleeve+gastrectomy+(SADI-S),+single+anastomosis+sleeve+ileal+(SASI)+bypass+with+sleeve+gastrectomy,+and+sleeve+gastrectomy+using+a+rodent+model+with+diabetes.">Comparison of the outcomes of single anastomosis duodeno-ileostomy with sleeve gastrectomy (SADI-S), single anastomosis sleeve ileal (SASI) bypass with sleeve gastrectomy, and sleeve gastrectomy using a rodent model with diabetes.</a> Obesity Surgery. 32 (4), 1209-1215 (2022).</li><li>Laura, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Establishing+a+reproducible+murine+animal+model+of+single+anastomosis+duodenoileal+bypass+with+sleeve+gastrectomy+(SADl-S).">Establishing a reproducible murine animal model of single anastomosis duodenoileal bypass with sleeve gastrectomy (SADl-S).</a> Obesity Surgery. 28 (7), 2122-2125 (2018).</li><li>Meoli, L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Intestine-specific+overexpression+of+LDLR+enhances+cholesterol+excretion+and+induces+metabolic+changes+in+male+mice.">Intestine-specific overexpression of LDLR enhances cholesterol excretion and induces metabolic changes in male mice.</a> Endocrinology. 160 (4), 744-758 (2019).</li><li>Abu El Haija, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Toll-like+receptor+4+and+myeloid+differentiation+factor+88+are+required+for+gastric+bypass-induced+metabolic+effects.">Toll-like receptor 4 and myeloid differentiation factor 88 are required for gastric bypass-induced metabolic effects.</a> Surgery for Obesity and Related Diseases. 17 (12), 1996-2006 (2021).</li><li>Kumar, S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Lipocalin-type+prostaglandin+D2+synthase+(L-PGDS)+modulates+beneficial+metabolic+effects+of+vertical+sleeve+gastrectomy.">Lipocalin-type prostaglandin D2 synthase (L-PGDS) modulates beneficial metabolic effects of vertical sleeve gastrectomy.</a> Surgery for Obesity and Related Diseases. 12 (8), 1523-1531 (2016).</li><li>Heffron, S. P., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Changes+in+lipid+profile+of+obese+patients+following+contemporary+bariatric+surgery:+A+meta-analysis.">Changes in lipid profile of obese patients following contemporary bariatric surgery: A meta-analysis.</a> The American Journal of Medicine. 129 (9), 952-959 (2016).</li><li>Carswell, K. A., Belgaumkar, A. P., Amiel, S. A., Patel, A. G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+systematic+review+and+meta-analysis+of+the+effect+of+gastric+bypass+surgery+on+plasma+lipid+levels.">A systematic review and meta-analysis of the effect of gastric bypass surgery on plasma lipid levels.</a> Obesity Surgery. 26 (4), 843-855 (2016).</li><li>Surve, A., Zaveri, H., Cottam, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Retrograde+filling+of+the+afferent+limb+as+a+cause+of+chronic+nausea+after+single+anastomosis+loop+duodenal+switch.">Retrograde filling of the afferent limb as a cause of chronic nausea after single anastomosis loop duodenal switch.</a> Surgery for Obesity and Related Diseases. 12 (4), 39-42 (2016).</li><li>Uysal, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Caecum+location+in+laboratory+rats+and+mice:+an+anatomical+and+radiological+study.">Caecum location in laboratory rats and mice: an anatomical and radiological study.</a> Laboratory Animals. 51 (3), 245-255 (2017).</li><li>S&#225;nchez-Pernaute, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Single-anastomosis+duodeno-ileal+bypass+with+sleeve+gastrectomy:+metabolic+improvement+and+weight+loss+in+first+100+patients.">Single-anastomosis duodeno-ileal bypass with sleeve gastrectomy: metabolic improvement and weight loss in first 100 patients.</a> Surgery for Obesity and Related Diseases. 9 (5), 731-735 (2013).</li><li>Wei, J. H., Yeh, C. H., Lee, W. J., Lin, S. J., Huang, P. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Sleeve+gastrectomy+in+mice+using+surgical+clips.">Sleeve gastrectomy in mice using surgical clips.</a> Journal of Visualized Experiments. (165), e60719 (2020).</li><li>Ying, L. D., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Technical+feasibility+of+a+murine+model+of+sleeve+gastrectomy+with+ileal+transposition.">Technical feasibility of a murine model of sleeve gastrectomy with ileal transposition.</a> Obesity Surgery. 29 (2), 593-600 (2019).</li><li>Bruinsma, B. G., Uygun, K., Yarmush, M. L., Saeidi, N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Surgical+models+of+Roux-en-Y+gastric+bypass+surgery+and+sleeve+gastrectomy+in+rats+and+mice.">Surgical models of Roux-en-Y gastric bypass surgery and sleeve gastrectomy in rats and mice.</a> Nature Protocols. 10 (3), 495-507 (2015).</li></ol>

减肥手术的技术不断发展，似乎是目前肥胖和相关代谢合并症最有效的治疗方法3，19，20。SADI-S手术于2007年首次描述4，是一种有前途的手术，与其他吸收不良手术相比，具有更大的代谢作用。动物模型，特别是允许快速生成转基因模型的小鼠，迫切需要充分了解这些改进背后的机制。在这里，我们描述了小?...

肥胖是一种新兴的流行情况，患病率日益增加，全世界每20名成年人中约有1人受到影响1。近年来，减肥手术已成为受影响成年人最有效的治疗选择，改善了体重减轻和代谢紊乱2，3，结果因所用外科手术的类型而异。
减肥手术的效果主要有两种机制：旨在增加饱腹感的限制（例如在袖状胃切除术（SG）中，80%的胃被切除）和吸收不良。在暗示限制和吸收不良的手术中，单吻合十二指肠回肠旁路术联合袖状胃切除术 （SADI-S） 已被提出作为 Roux-en-Y 胃旁路术 （RYGB） 的替代方案，其中在大约 20% 的患者中观察到体重恢复4，5。在这种技术中，袖状胃切除术与小肠重新排列有关，将其分为胆道和短公共肢体（占小肠总长度的三分之一）（图1A）。从技术上讲，SADI-S 比 RYGB 具有仅需要一次吻合的优势，可将手术时间缩短约 30%。此外，这种方法保留了幽门，有助于降低消化性溃疡疾病的风险并限制吻合口渗漏。SADI-S还与高代谢改善率有关，在过去几年中强烈支持其使用6，7。
由于代谢效应已成为减肥手术的基础，阐明其机制似乎至关重要。因此，使用动物模型进行减肥手术对于更好地了解其代谢作用以及所涉及的细胞和分子途径至关重要8。例如，这些模型有助于更好地了解SG或RYGB在受控环境中9后食物摄入量的变化，以及通过肠道屏障10，11研究葡萄糖或胆固醇通量;这些信息在临床研究中很少可用。这些知识可以帮助确定他们的最佳手术适应症。我们之前描述了SG和RYGB12的小鼠模型。然而，尽管SADI-S在临床实践中取得了有希望的结果，但它仅在大鼠13，14，15中开发和描述。然而，鉴于其遗传延展性，小鼠模型过去可用于研究此类程序的各种代谢效应16，17，18，并且SADI-S小鼠模型可用于评估SADI-S的影响，尽管技术困难。
在本文中，我们以可重复的方式描述了SADI-S程序在小鼠中的适应性（图1B）。特别注意围手术期护理的描述。

<table border="1" fo:keep-together.within-page="1" fo:keep-with-next.within-page="always">
	<tbody>
		<tr>
			<td>Agagani needle 26 G</td>
			<td>Terumo</td>
			<td>050101B</td>
			<td>26 G needle</td>
		</tr>
		<tr>
			<td>Betadine dermique&#160;</td>
			<td>Pharma-gdd</td>
			<td>3300931499787</td>
			<td>Povidone solution</td>
		</tr>
		<tr>
			<td>Betadine scrub</td>
			<td>Pharma-gdd</td>
			<td>&#160;3400931499787</td>
			<td>Povidone solution</td>
		</tr>
		<tr>
			<td>Binocular microscope</td>
			<td>Optika Microscopes Italy</td>
			<td>SZN-9</td>
			<td>Binocular stereomicroscope</td>
		</tr>
		<tr>
			<td>Buprecare</td>
			<td>Animalcare</td>
			<td>3760087151244</td>
			<td>Buprenorphin</td>
		</tr>
		<tr>
			<td>Castroviejo, straight 9 cm</td>
			<td>F.S.T</td>
			<td>12060-02</td>
			<td>Micro scissors</td>
		</tr>
		<tr>
			<td>Castroviejo, straight 9 cm</td>
			<td>F.S.T</td>
			<td>12060-02</td>
			<td>Needle holder</td>
		</tr>
		<tr>
			<td>Chlorure de sodium Fresenius 0.9%</td>
			<td>Fresenius Kabi&#160;</td>
			<td>BE182743</td>
			<td>NaCl 0.9%</td>
		</tr>
		<tr>
			<td>Clamoxyl</td>
			<td>Med&#39;vet</td>
			<td>5414736007496</td>
			<td>Amoxicilline</td>
		</tr>
		<tr>
			<td>Cotton buds</td>
			<td>Comed</td>
			<td>2510805</td>
			<td>Cotton swabs</td>
		</tr>
		<tr>
			<td>Element HT5</td>
			<td>Scilvet</td>
			<td>Element HT5</td>
			<td>Automated hematology analyzer</td>
		</tr>
		<tr>
			<td>Emeprid</td>
			<td>CEVA</td>
			<td>3411111914365</td>
			<td>Metoclopramid</td>
		</tr>
		<tr>
			<td>Extra Fine Graefe Forceps, curved (tip width: 0.5 mm)</td>
			<td>F.S.T</td>
			<td>11152-10</td>
			<td>Surgical forceps</td>
		</tr>
		<tr>
			<td>Extra Fine Graefe Forceps, straight (tip width: 0.5 mm)</td>
			<td>F.S.T</td>
			<td>11150-10</td>
			<td>Surgical forceps</td>
		</tr>
		<tr>
			<td>Fercobsang</td>
			<td>Vetoprice</td>
			<td>QB03AE04</td>
			<td>Iron, multivitamins and minerals&#160;</td>
		</tr>
		<tr>
			<td>Forane</td>
			<td>Baxter</td>
			<td>1001936060</td>
			<td>Isoflurane</td>
		</tr>
		<tr>
			<td>Graefe forceps, straight (tip width: 0.8 mm)</td>
			<td>F.S.T</td>
			<td>11050-10</td>
			<td>Forceps</td>
		</tr>
		<tr>
			<td>Graphpad Prism version 8.0</td>
			<td>GraphPad Software, Inc.</td>
			<td>Version 8.0</td>
			<td>Software for statistical analysis</td>
		</tr>
		<tr>
			<td>Heat pad</td>
			<td>Intellibio innovation</td>
			<td>A-2101-00300</td>
			<td>Heat pad</td>
		</tr>
		<tr>
			<td>Incubator</td>
			<td>Bioconcept Technologies</td>
			<td>Manufactured on demand</td>
			<td>Incubator&#160;</td>
		</tr>
		<tr>
			<td>Lighting</td>
			<td>Optika Microscopes Italy</td>
			<td>CL-30</td>
			<td>Lighting for microscopy</td>
		</tr>
		<tr>
			<td>Ocrygel</td>
			<td>Med&#39;vet</td>
			<td>3700454505621</td>
			<td>Carboptol 980 NF</td>
		</tr>
		<tr>
			<td>Pangen 2.5 cm x 3.5 cm</td>
			<td>Urgovet</td>
			<td>A02978</td>
			<td>Haemostatic collagen compress</td>
		</tr>
		<tr>
			<td>Prolene 6/0</td>
			<td>B.Braun</td>
			<td>3097915</td>
			<td>Optilene 6/0 (0.7 metric) 75 cm 2XDR13 CV2 RCP, suture cord</td>
		</tr>
		<tr>
			<td>Prolene 8/0</td>
			<td>Ethicon</td>
			<td>8732</td>
			<td>2 x BV175-6 MP, 3/8 Circle, 8 mm,&#160; suture cord</td>
		</tr>
		<tr>
			<td>Scissors</td>
			<td>F.S.T</td>
			<td>146168-09</td>
			<td>Surgical scissors</td>
		</tr>
		<tr>
			<td>Sterile compresses&#160;</td>
			<td>Laboartoire Sylamed</td>
			<td>211S05-50</td>
			<td>Non-woven sterile compressed</td>
		</tr>
		<tr>
			<td>Terumo Syringe</td>
			<td>Terumo</td>
			<td>50828</td>
			<td>1 mL syringe</td>
		</tr>
		<tr>
			<td>Titanium hemostatic clip</td>
			<td>P&#233;ters Surgical</td>
			<td>B2180-1</td>
			<td>Surgical clip</td>
		</tr>
		<tr>
			<td>Vannas Wolff</td>
			<td>F.S.T</td>
			<td>15009-08</td>
			<td>Micro scissors</td>
		</tr>
		<tr>
			<td>Vita Rongeur</td>
			<td>Virbac</td>
			<td>3597133087611</td>
			<td>Vitamin supplementation</td>
		</tr>
		<tr>
			<td>Vitaltec stainless</td>
			<td>P&#233;ters Surgical</td>
			<td>PB 220-EB Medium</td>
			<td>Surgical clip applier</td>
		</tr>
	</tbody>
</table>

single-anastomosis duodeno-ileal bypass with sleeve gastrectomy model in mice

肥胖是世界范围内的主要健康问题。作为应对措施，减肥手术已经出现，通过限制性和吸收不良机制治疗肥胖及其相关合并症（例如糖尿病、血脂异常、非酒精性脂肪性肝炎、心血管事件和癌症）。了解这些程序允许这种改进的机制通常需要将它们转移到动物身上，特别是在小鼠中，因为很容易产生转基因动物。最近，单吻合十二指肠回肠旁路术与袖状胃切除术（SADI-S）已成为一种同时使用限制性和吸收不良作用的手术，在严重肥胖的情况下被用作胃旁路术的替代方案。到目前为止，该程序与强大的代谢改善有关，这导致其在日常临床实践中的使用显着增加。然而，由于缺乏动物模型，这些代谢效应背后的机制研究得很少。在本文中，我们提出了一种可靠且可重复的小鼠SADI-S模型，特别关注围手术期管理。这种新的啮齿动物模型的描述和使用将有助于科学界更好地了解SADI-S诱导的分子，代谢和结构变化，并更好地确定临床实践的手术适应症。

学习曲线 该模型的学习曲线如图 6 所示。观察到手术时间逐渐减少，在4周的强化训练后达到约60分钟的手术（图6A）。术后5天生存率也随着时间的推移而改善，在常规练习期间达到77%（图6B）。最常见的死亡原因是吻合口瘘和导致胆道腹膜炎的传入袢综合征。我们在第一个月晚些时候用本手稿中描述的技术观察到?...

Watch this Scientific Journal Video about Single-Anastomosis Duodeno-Ileal Bypass with Sleeve Gastrectomy Model in Mice at JoVE.com

Single-Anastomosis Duodeno-Ileal Bypass with Sleeve Gastrectomy Model in Mice

单吻合十二指肠回肠旁路术 （SADI-S） 是一种新兴的减肥手术，具有重要的代谢作用。在本文中，我们提出了一种可靠且可重复的小鼠SADI-S模型。

小鼠单吻合十二指肠回肠旁路与袖状胃切除术模型

Obesity is a major health issue worldwide. As a response, bariatric surgeries have emerged to treat obesity and its related comorbidities (e.g., diabetes ...

因此，该协议是小鼠CDS的首次描述，使我们能够模拟临床实践中进行的手术，并以更可预测的方式破译代谢细胞和分子机制。与胃旁路术相比，该手术需要的吻合更少，因此手术时间缩短，存活率提高，代谢益处与胃旁路术相似。首先皮下注射丁丙诺啡、阿莫西林、甲氧氯普胺和铁剂。从剑突过程中，使用电动剃须刀剃除小鼠腹部的前三分之二，并使用碘聚维酮溶液对小鼠腹部进行两次消毒。然后将鼠标仰卧放在覆盖有干净垫子的专用加热垫上。使用脚趾捏测检查麻醉深度，并用无菌保鲜膜覆盖鼠标。接下来，通过固定下核心并将一毫升注射器或等效物放在鼠标背后来过度伸展鼠标的腹部。在无菌敷料中切开一个窗口大小的开口作为未来的切口部位，并将其用作覆盖鼠标的操作区域。用剪刀进行正中剖腹手术，将腹部皮肤从剑突打开到腹部中部。然后用剪刀沿着腹肌之间的线性白肌打开腹壁。使用湿润的棉签，轻轻地从腹腔中移动十二指肠，以查看其前侧和后侧。然后定位主胆管，在小网膜和十二指肠后侧的双目显微镜下立即可见。在双目显微镜下，从十二指肠动脉之间的主胆管中观察大约一个区域，并使用弯曲的微镊子从十二指肠的一侧到另一侧穿透该区域。接下来，使用 6.0 不可吸收缝合线在动脉之间进行十二指肠否定。使用湿润的棉签和非创伤夹从腹腔中动员胃，并使用微型剪刀将其与周围器官分开。然后分离大网膜，切开胃和脾之间的短胃动脉和连接胃和食道下部的脂肪瘤。使用微型剪刀，通过打开眼底并使用棉签去除残留的食物来进行五毫米的胃切开术。沿着胃的较大曲率应用手术夹以排除大约 80% 的胃，并通过用微型剪刀剪开排除的胃来去除。现在，通过从胃切除术的开始到结束进行运行缝合，将手术夹固定到一定的不渗透性。在双目显微镜下，观察位于盲肠前的最后一个回肠袢。从最后一个回肠袢轻轻地将腹腔外的小肠活动起来，并处理小肠，使最后一个回肠袢位于左侧。使用先前尺寸的缝合线，从最后一个回肠袢处测量 10 厘米。为了确保未来的胆道支从其左侧到达吻合侧，请在未来吻合口的一侧周围做一个大的小肠环。此时，使用微型剪刀打开小肠进行四毫米的小肠切开术。在胃和之前进行的结扎术之间的幽门之后立即对十二指肠进行四毫米切开术。然后放置可吸收的止血胶原蛋白敷料以利于体内平衡。使用不可吸收的 8.0 缝合线，从后侧到前侧进行左右十二指肠回鼻腺吻合术，并按照手稿中所述在腹腔内显示小肠。使用一毫升注射器将小鼠注入腹腔，用500微升37摄氏度盐水溶液对小鼠进行再水化。现在使用单条6.0不可吸收的跑步缝合线来闭合肌肉腱膜神经质层，并使用6.0不可吸收的分离缝合线闭合腹部皮肤。经过四周的强化训练，观察到手术时间逐渐减少，达到约60分钟。术后五天的生存率也随着时间的推移而提高，在常规练习中达到77%。从术后第四天开始，与假小鼠相比，SADI-S小鼠的体重明显减轻。在SADI-S小鼠中观察到每日食物摄入量显着增加。然而，补铁后，Sham组和SADI-S组之间的血红蛋白浓度没有显着差异。进行吻合术时，至关重要的是要以胆汁和食物不相互对立的方式处理肠道，以避免传入环综合征。

Research

JoVE Journal

Medicine

Modeling Stroke in Mice - Middle Cerebral Artery Occlusion with the Filament Model

建模在小鼠体内的行程 - 中东与灯丝模型大脑中动脉闭塞

Stroke is among the most frequent causes of death and adult disability, especially in highly developed countries. However, treatment options to date are ...

科研

医学

Intraluminal Middle Cerebral Artery Occlusion (MCAO) Model for Ischemic Stroke with Laser Doppler Flowmetry Guidance in Mice

Intraluminal Middle Cerebral Artery Occlusion MCAO Model for Ischemic Stroke with Laser Doppler Flowmetry Guidance in Mice

腔内中东大脑中动脉闭塞（MCAO）缺血性中风模型在小鼠体内的激光多普勒血流指导

Stroke is the third leading cause of death and the leading cause of disability in the world, with an estimated cost of near $70 billion 
in the United ...

Roux-en-Y Gastric Bypass Operation in Rats

空肠Roux-en-Y胃搭桥手术大鼠

Currently, the most effective therapy for the treatment of morbid obesity to induce significant and maintained body weight loss with a proven mortality ...

Evaluation of a Novel Laser-assisted Coronary Anastomotic Connector - the Trinity Clip - in a Porcine Off-pump Bypass Model

评价一种新型激光辅助冠状动脉吻合口连接器 - 三位一体夹 - 在猪非体外循环旁路模式

To simplify and facilitate beating heart (i.e., off-pump), minimally invasive coronary artery bypass surgery, a new coronary anastomotic connector, the ...

Vein Interposition Model: A Suitable Model to Study Bypass Graft Patency

静脉介入型号：研究旁路移植通畅一个合适的模型

Bypass grafting is an established treatment method for coronary artery disease. Graft patency continues to be the Achilles heel of saphenous vein grafts. ...

Techniques of Sleeve Gastrectomy and Modified Roux-en-Y Gastric Bypass in Mice

袖胃切除术的技术和改良的小鼠空肠Roux-en-Y胃旁路

Obesity is a major public health issue, with a prevalence of 4 to 28% for men and 6.2 to 36.5% for women in Europe (from 2003 to 2008). Morbid obesity is ...

Using the Sleeve Technique in a Mouse Model of Aortic Transplantation - An Instructional Video

套筒技术在主动脉移植小鼠模型中的应用-教学视频

Orthotopic aortic transplantation using the sleeve technique reduces injury to the aorta with failure rate of only 10-20%. The time to anastomose the ...

Cardiopulmonary Bypass in a Mouse Model: A Novel Approach

小鼠体外循环模型: 一种新方法

As prolonged cardiopulmonary bypass becomes more essential during cardiac interventions, an increasing clinical demand arises for procedure optimization ...

Isometric Contractility Measurement of the Mouse Mesenteric Artery Using Wire Myography

用线 Myography 测定小鼠肠系膜动脉的等轴收缩力

The wire myograph technique is used to assess the contractility of vascular smooth muscles in response to depolarization, GPCR agonists/inhibitors and ...

One-anastomosis Gastric Bypass (OAGB) in Rats

One-anastomosis Gastric Bypass OAGB in Rats

大鼠胃旁路 (oagb) 的一次吻合

The goal of this protocol is to set up a preclinical model of bariatric surgery and, more specifically, OAGB in obese rats. Based on this preclinical ...