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09:46 min
February 17th, 2023
DOI :
10.3791/64633-v
Chapters
0:04
Introduction
0:52
Preparation of Pre‐Gel Solution for the Stroma Equivalent
2:24
Surface Micropatterning
3:24
Seeding Epithelial Cells on the Stromal Equivalent
4:40
Establishing Air‐Liquid Interface (ALI)
5:32
Endothelial Cell Seeding
6:24
Reconnect the Chip to Flow
7:02
Results: Analysis of Human Epithelial Tissues on an Open‐Top Organ‐Chip
8:49
Conclusion
Transcript
传统的扩散培养方法往往无法概括活器官的复杂环境,导致组织特异性标志物和功能的丧失。我们开发了一种开顶芯片,结合了扩散培养和微流体原理,以密切模拟上皮组织的微环境。通过包括天然存在于活体器官中但经常被传统体外模型忽视的生化和生物力学线索,开顶芯片代表了封闭系统之间的更好选择。
今天演示该程序的是Adya Panchal,我实验室的一名研究生。首先,将所需的试剂放在冰上的生物安全柜下。培养组织特异性间充质细胞以获得80%至90%的汇合度,然后根据制造商的建议使用胰蛋白酶或其他方法解
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Summary
本协议描述了开顶器官芯片在成功建立和成熟原代组织(皮肤、肺泡、气道和肠)的全层器官芯片培养物方面的能力和基本培养方式,提供了研究人上皮/间充质和血管生态 位界面的不同功能方面的机会体外。
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