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DOI :
10.3791/64662-v
•
9:11 min
April 28th, 2023
Chapters
0:05
Introduction
0:53
Drosophila Embryo Fixation and Devitellinization
3:04
Preparing PDMS Wells
3:57
Adhering the Embryos to the Coverslips
4:33
Activation and Gelation
5:48
Digestion and Expansion
6:33
Mounting and Imaging
7:08
Results: Analysis of Unexpanded and Expanded Embryos
8:45
Conclusion
여기에서는 기존의 레이저 스캐닝 컨포칼 현미경을 사용하여 초고해상도 이미징을 달성하기 위해 초기 초파리 배아에서 확장 현미경을 구현하기 위한 프로토콜을 제시합니다.
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