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DOI :
10.3791/64682-v
December 2nd, 2022
Chapters
0:05
Introduction
0:49
Prepare Palmitic Acid Working Solution
1:27
Applying Palmitic Acid Coating to Microtray
2:53
Loading the Microtray with Low‐Melt Nematode Growth Media (lmNGM) and Seeding the Microtray Wells with Bacterial Food
3:47
Enclosing Each Microtray Inside a Single‐Well Plate
4:25
Adding Worms to the Microtray and Preparing the Culture Environment for Long‐Term Use
5:01
Imaging Individual Worms in Microtray Wells
5:27
Results: Correlation of Phenotypes in Individual Animals Across Populations
6:53
Conclusion
ここでは、生涯にわたる生理学的パラメータの追跡と蛍光定量のために、単離された個々の線虫を固体培地上で培養するためのプロトコルを紹介します。この培養システムには、動物が逃げるのを防ぐために、シングルワームウェルの周りにパルミチン酸バリアが含まれており、病原菌や化学的ストレッサーなどの嫌悪的介入を使用できます。
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