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DOI :
10.3791/64698-v
•
11:52 min
January 27th, 2023
Chapters
0:04
Introduction
0:56
Isolation of Peripheral Blood Mononuclear Cells (PBMCs) from Whole Blood
2:34
Enrichment of CD14+ Monocytes from PBMCs
4:20
Osteoclast Differentiation In Vitro
5:46
TRAP Staining for Osteoclasts
7:10
Actin Ring Fluorescent Staining
9:40
Results: Differentiation of Osteoclasts and Assessing the Function of Mature Osteoclasts
11:12
Conclusion
破骨細胞は、体内の重要な骨吸収細胞です。このプロトコルは、ヒト末梢血単球からの破骨細胞の in vitro 分化のための信頼できる方法を記載しています。この方法は、恒常性や疾患における破骨細胞の生物学をさらに理解するための重要なツールとして使用できます。
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