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DOI :
10.3791/64707-v
•
9:03 min
March 17th, 2023
Chapters
0:04
Introduction
1:01
Closed Semi‐Automated WJ‐hMSC Dissociation and Harvesting Using Closed Counterflow Centrifugation
3:56
Running the Protocol
6:03
Results: Characterizing the WJ‐MSCs Harvested Using the Closed Semi‐Automated Counterflow Centrifugation
8:15
Conclusion
在这里,我们提出了一种使用逆流离心系统以封闭的半自动方式从多层烧瓶中收获贴壁细胞的方案。该协议可用于从其他细胞扩增平台收获贴壁和悬浮细胞,只需对现有步骤进行少量修改。
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