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DOI :
10.3791/64707-v
•
9:03 min
March 17th, 2023
Chapters
0:04
Introduction
1:01
Closed Semi‐Automated WJ‐hMSC Dissociation and Harvesting Using Closed Counterflow Centrifugation
3:56
Running the Protocol
6:03
Results: Characterizing the WJ‐MSCs Harvested Using the Closed Semi‐Automated Counterflow Centrifugation
8:15
Conclusion
ここでは、向流遠心分離システムを使用して、多層フラスコから接着細胞を密閉半自動方式で回収するためのプロトコルを紹介します。このプロトコルは、既存のステップにほとんど変更を加えることなく、他の細胞増殖プラットフォームから接着細胞と浮遊細胞の両方を回収するために適用できます。
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