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Generation of Human Blood Vessel Organoids from Pluripotent Stem Cells

5.9K Views

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09:46 min

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January 20th, 2023

DOI :

10.3791/64715-v

January 20th, 2023

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Blood Vessel Organoids

Chapters in this video

0:04

Introduction

0:52

Day 0: Generation of Pluripotent Aggregates from Single-Cell Suspension

2:34

Day 1: Mesoderm Induction of the Aggregates

3:41

Day 4: Vascular Induction and Priming of the Aggregates

4:20

Day 6: Aggregate Embedding and Vessel Sprout Induction

6:02

Day 11: Isolation and Maturation of the Blood Vessel Organoids (BVOs)

7:25

Results: Stepwise Progression of Human Blood Vessel Organoid Generation from hPSCs

9:13

Conclusion

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