Seeding, Cell Treatment, and Preparation of Cell Lysate
2:10
BCA Assay for Protein Quantification
2:49
Population-Based Assay for Caspase-3/7 Activity
3:31
Single-Cell Assay for Caspase-3/7 Activity
4:07
Results: Smac Mimetic-Induced Effect on Caspase-3/7 Activity
4:50
Conclusion
Denne protokol beskriver to metoder til måling af caspaseaktivitet gennem et fluorogent substrat ved hjælp af flowcytometri eller et spektrofluorometer.