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Non-Aqueous Isolation and Enrichment of Glandular Capitate Stalked and Sessile Trichomes from Cannabis sativa

DOI :

10.3791/64798-v

May 12th, 2023

May 12th, 2023

1,530 Views

1Department of Vegetable Research, ARO-Volcani Center, 2Department of Vegetable Research, ARO-Newe Ya‘ar Center, 3Department of Ornamental Horticulture and Biotechnology, Institute of Plant Sciences, ARO- Volcani Center, 4Department of Fruit Science, ARO- Volcani Center

A protocol is presented for the convenient and high-throughput isolation and enrichment of glandular capitate stalked and sessile trichomes from Cannabis sativa. The protocol is based on a dry, non-buffer extraction of trichomes using only liquid nitrogen, dry ice, and nylon sieves and is suitable for RNA extraction and transcriptomic analysis.

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