Non-Aqueous Isolation and Enrichment of Glandular Capitate Stalked and Sessile Trichomes from Cannabis sativaShahar Cohen 1, Maxim Itkin 1, Adi Faigenboim 1, Rachel Davidovich-Rikanati 2, Einat Bar 2, Daniel Hasson 1, Nurit Shalev 3, Hinanit Koltai 3, Oded Sagee 4, Efraim Lewinsohn 2, Ben Spitzer-Rimon 3, Arthur A. Schaffer 1
1Department of Vegetable Research, ARO-Volcani Center, 2Department of Vegetable Research, ARO-Newe Ya‘ar Center, 3Department of Ornamental Horticulture and Biotechnology, Institute of Plant Sciences, ARO- Volcani Center, 4Department of Fruit Science, ARO- Volcani Center
A protocol is presented for the convenient and high-throughput isolation and enrichment of glandular capitate stalked and sessile trichomes from Cannabis sativa. The protocol is based on a dry, non-buffer extraction of trichomes using only liquid nitrogen, dry ice, and nylon sieves and is suitable for RNA extraction and transcriptomic analysis.
