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DOI :
10.3791/64810-v
January 20th, 2023
Chapters
0:05
Introduction
0:50
Pre-Formulation Preparation
1:51
Preparation of the Lipid and Nucleic Acid Mixes
2:33
Microfluidic Formulation of mRNA-LNPs
3:56
Post-Formulation Processing and Characterization of the mRNA-LNPs
4:51
In Vitro Transfection of HepG2 Cells
5:55
In Vivo Evaluation of mRNA-LNPs in Mice Following Tail Vein Injection
7:34
Results: Formulation of mRNA-LNPs and the Assessment of In Vitro and In Vivo Performance
8:24
Conclusion
ここでは、ホタルルシフェラーゼをコードするmRNAをカプセル化する脂質ナノ粒子(LNP)を製剤化するためのプロトコルが提示されます。これらのLNPは、in vitro でHepG2細胞で、in vivo でC57BL/6マウスでその効力を試験しました。
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