Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells

3.6K Views

10:34 min

December 9th, 2022

DOI :

10.3791/64820-v

December 9th, 2022


Transcript

Explore More Videos

RNA Structure

Chapters in this video

0:04

Introduction

0:36

Gene‐Specific In Vitro DMS Modification

2:49

Gene‐Specific RT‐PCR of Modified RNA

4:19

Whole‐Genome DMS‐MaP Using Virus‐Infected Cells

7:17

Analysis of the Sequencing Data

8:12

Results: Probing RNA Structure with Dimethyl Sulfate Mutational Profiling and Sequencing

10:00

Conclusion

Related Videos

article

09:18

Time-resolved ElectroSpray Ionization Hydrogen-deuterium Exchange Mass Spectrometry for Studying Protein Structure and Dynamics

9.7K Views

article

09:51

Investigating Protein Sequence-structure-dynamics Relationships with Bio3D-web

15.2K Views

article

14:32

Polysome Profiling in Leishmania, Human Cells and Mouse Testis

17.7K Views

article

08:58

Characterization of Glycoproteins with the Immunoglobulin Fold by X-Ray Crystallography and Biophysical Techniques

12.4K Views

article

09:52

Probing The Structure And Dynamics Of Nucleosomes Using Atomic Force Microscopy Imaging

11.5K Views

article

07:04

Site-Directed Mutagenesis for In Vitro and In Vivo Experiments Exemplified with RNA Interactions in Escherichia Coli

19.6K Views

article

00:15

Interactions with and Membrane Permeabilization of Brain Mitochondria by Amyloid Fibrils

5.9K Views

article

00:11

Extremely Rapid and Specific Metabolic Labelling of RNA In Vivo with 4-Thiouracil (Ers4tU)

10.6K Views

article

10:12

Rapid and Cost-Effective RNA Extraction of Rat Pancreatic Tissue

9.1K Views

article

05:47

Phosphoproteomic Strategy for Profiling Osmotic Stress Signaling in Arabidopsis

5.1K Views

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2025 MyJoVE Corporation. All rights reserved