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Site-Directed Mutagenesis for In Vitro and In Vivo Experiments Exemplified with RNA Interactions in Escherichia Coli

DOI :

10.3791/58996-v

February 5th, 2019

February 5th, 2019

19,018 Views

1Department of Biochemistry and Molecular Biology, University of Southern Denmark

Site-directed mutagenesis is a technique used to introduce specific mutations in deoxyribonucleic acid (DNA). This protocol describes how to do site-directed mutagenesis with a 2-step and 3-step polymerase chain reaction (PCR) based approach, which is applicable to any DNA fragment of interest.

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Site directed Mutagenesis

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