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DOI :
10.3791/64848-v
•
8:47 min
December 16th, 2022
Chapters
0:04
Introduction
0:50
Seeding of Cryopreserved Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes (hiPSC-CMs)
1:25
Dissociation and Counting of the Pre-Plated hiPSC-CMs
2:49
Flexible Hydrogel Substrates Preparation
4:35
Contraction Recording and Analysis
6:52
Results: Evaluation of CCM in 2D Human Stem Cell-Derived Cardiomyocytes
7:59
Conclusion
在这里,我们展示了一种非侵入性心脏医疗设备收缩力评估方法,该方法使用2D人诱导多能干细胞衍生心肌细胞(hiPSC-CM)单层,接种在柔性基板上,并结合基于视频的显微镜。该工具将可用于心脏电生理学装置收缩特性的 体外 评估。
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