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DOI :
10.3791/64848-v
•
8:47 min
December 16th, 2022
Chapters
0:04
Introduction
0:50
Seeding of Cryopreserved Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes (hiPSC-CMs)
1:25
Dissociation and Counting of the Pre-Plated hiPSC-CMs
2:49
Flexible Hydrogel Substrates Preparation
4:35
Contraction Recording and Analysis
6:52
Results: Evaluation of CCM in 2D Human Stem Cell-Derived Cardiomyocytes
7:59
Conclusion
本研究では、2次元ヒト人工多能性幹細胞由来心筋細胞(hiPSC-CM)単層をフレキシブル基板上にめっきし、ビデオベースの顕微鏡と組み合わせて、非侵襲的心臓医療機器の収縮性評価方法を実証する。このツールは、心臓電気生理学デバイスの収縮特性の in vitro 評価に役立ちます。
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