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DOI :
10.3791/64913-v
April 7th, 2023
Chapters
0:04
Introduction
1:01
Growing the Plants
1:43
Generating the Protoplasts
2:43
Fluorescence‐Activated Cell Sorting (FACS) of the Protoplasts
4:23
Smart‐Seq2 Library Preparation
7:00
Results: Protoplast Isolation and Transcriptome Analysis
8:11
Conclusion
고처리량 scRNA-seq 분석법의 실현 가능성과 효과는 식물 연구에서 단일 세포 시대를 예고합니다. 여기에 제시된 것은 특정 Arabidopsis thaliana 뿌리 세포 유형을 분리하고 후속 전사체 라이브러리 구성 및 분석을 위한 강력하고 완전한 절차입니다.
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