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DOI :
10.3791/64917-v
•
8:19 min
February 10th, 2023
Chapters
0:04
Introduction
0:50
Establishment of Piglet Intestinal 3D Organoids from Frozen Epithelial Crypts
1:52
Passage of Piglet Intestinal 3D Organoids Derived from Frozen Crypts
3:54
Culture of Cell Monolayers Derived from 3D Organoids
6:17
Results: Characteristics of Cell Monolayers Derived from Pig Intestinal 3D Organoids
7:37
Conclusion
在这里,我们描述了从冷冻保存的上皮隐窝培养猪肠道3D类器官的方案。我们还描述了一种建立源自3D类器官的细胞单层的方法,允许进入上皮细胞的顶端。
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