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DOI :
10.3791/64917-v
•
8:19 min
February 10th, 2023
Chapters
0:04
Introduction
0:50
Establishment of Piglet Intestinal 3D Organoids from Frozen Epithelial Crypts
1:52
Passage of Piglet Intestinal 3D Organoids Derived from Frozen Crypts
3:54
Culture of Cell Monolayers Derived from 3D Organoids
6:17
Results: Characteristics of Cell Monolayers Derived from Pig Intestinal 3D Organoids
7:37
Conclusion
여기에서는 냉동보존된 상피 선와에서 돼지 장 3D 오가노이드를 배양하는 프로토콜을 설명합니다. 우리는 또한 3D 오가노이드에서 파생된 세포 단층을 확립하여 상피 세포의 정점 측면에 접근할 수 있도록 하는 방법을 설명합니다.
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