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DOI :
10.3791/64970-v
•
8:55 min
February 17th, 2023
Chapters
0:05
Introduction
0:43
Preparation of Lipids and Agarose Hydrogel
1:33
PMMA Chamber Assembly and Lipid Monolayer Formation
3:06
Preparation of Lipid-Coated Aqueous Droplets in Hexadecane/Silicone Oil Solution
4:17
Preparation and Imaging of Single Ion Channels in DIB Membranes
6:49
Results: Isolation of TOM-CC and Correlation of Channel Activity with Lateral Mobility of TOM-CC
8:18
Conclusion
このプロトコルでは、TIRF顕微鏡を使用して個々のイオンチャネルを追跡し、支持された脂質膜におけるそれらの活性を決定し、それによって側方膜の動きとチャネル機能の間の相互作用を定義する方法について説明します。メンブレンの準備、データの記録、および結果の分析方法について説明します。
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