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DOI :
10.3791/64970-v
•
8:55 min
February 17th, 2023
Chapters
0:05
Introduction
0:43
Preparation of Lipids and Agarose Hydrogel
1:33
PMMA Chamber Assembly and Lipid Monolayer Formation
3:06
Preparation of Lipid-Coated Aqueous Droplets in Hexadecane/Silicone Oil Solution
4:17
Preparation and Imaging of Single Ion Channels in DIB Membranes
6:49
Results: Isolation of TOM-CC and Correlation of Channel Activity with Lateral Mobility of TOM-CC
8:18
Conclusion
이 프로토콜은 TIRF 현미경을 사용하여 개별 이온 채널을 추적하고 지지된 지질막에서 그 활성을 결정하여 측면 막 이동과 채널 기능 간의 상호 작용을 정의하는 방법을 설명합니다. 멤브레인을 준비하고, 데이터를 기록하고, 결과를 분석하는 방법을 설명합니다.
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