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09:45 min
March 17th, 2023
DOI :
10.3791/65032-v
Chapters
0:05
Introduction
1:34
Fabricating the Master Wafer and Preparing the Microfluidic Device
3:46
Making the PDMS-Coated Glass Slide and Bonding the Microfluidic Device
5:24
Surface Functionalization of the Microfluidic Device
6:48
Liposome Production
7:33
Results: Production of Monodispersed Liposomes and Subsequent pH-Triggered Liquid-Liquid Phase Separation of pLL/ATP Inside Liposomes
9:02
Conclusion
Transcript
OLA是一种对合成生物学界有用的多功能微流体平台。特别是对人造细胞进行生物工程。基于OLA的脂质体可以帮助我们理解生物学中的自组织原理并构建细胞模拟组装。
OLA以高通量方式生产单分散、单层、细胞大小的脂质体,并具有出色的包封效率。它需要非常小的样品体积,大约为50微升,并且形成的脂质体可立即用于进一步的片上实验。OLA可用于研究微约束内的生物反应,囊泡动力学和生物分子缩合物及其与膜的相互作用。
OLA还被用作药物筛选的高通量平台,例如,测试抗菌剂的渗透性和膜活性。
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Summary
本协议描述了辛醇辅助脂质体组装(OLA),这是一种产生生物相容性脂质体的微流体技术。OLA生产具有高效封装的单分散微米级脂质体,允许立即进行片上实验。预计该协议特别适用于合成生物学和合成细胞研究。
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