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JoVE Journal

Chemistry

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Standardized Identification of Compound Structure in Tibetan Medicine Using Ion Trap Mass Spectrometry and Multiple-Stage Fragmentation Analysis

747 Views

09:24 min

March 17th, 2023

March 17th, 2023

747 Views

0:04

Introduction

0:51

Abelmoschus manihot Seeds (AMS) Sample Preparation

1:32

Mass Spectrometry (MS) Setting

2:12

Liquid Chromatography Prerun, Method Establishment, and MS Acquisition

4:07

Operating Multiple-Stage Mass Spectrometry

6:10

Manual MSn Data Analysis

6:47

Results: Multiple-Stage Fragmentation and Structural Analysis of Unknown AMS Compounds

8:37

Conclusion

Transcript

This method can help researchers analyze the structures of Tibetan medicine compounds, especially the tree's components. It can provide insight into the qualitative study of compound's content in natural medicine. The main advantage of this techni

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Here, we describe a general protocol and design that could be applied to identify trace amounts and minor constituents in the complex natural product formulations (matrixes) in Tibetan medicine.

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