Name: a rabbit aortic valve stenosis model induced by direct balloon injury
Uploaded: 2023-03-31T14:40:00
Description: Watch this Scientific Journal Video about A Rabbit Aortic Valve Stenosis Model Induced by Direct Balloon Injury at JoVE.com

这项工作得到了韩国国家研究基金会（NRF）资助，由韩国政府（MSIT）（编号2020R1A4A3079570），教育部（编号2021R1I1A1A01051425）和工业战略技术发展计划（第20014873号）资助，由韩国贸易，工业和能源部资助。

所有动物研究程序均根据《实验动物福利法》、《实验动物护理和使用指南》以及韩国天主教大学医学院机构动物护理和使用委员会（IACUC）提供的《动物实验指南和政策》进行批准和执行（批准号： CUMC-2021-0176-05）。本研究使用体重 3.5-4.0 kg 的 3 个月大雄性新西兰白兔 （NZW），在标准条件下将其饲养在单独的笼子中。给兔子喂食正常饮食或补充有50,000U维生素D2的富含胆固醇的0.5%饮食（见 ...

<ol>
	<li>Aliev, G., Burnstock, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Watanabe+rabbits+with+heritable+hypercholesterolaemia:+A+model+of+atherosclerosis.">Watanabe rabbits with heritable hypercholesterolaemia: A model of atherosclerosis.</a> Histology and Histopathology. 13 (3), 797-817 (1998).</li><li>Cimini, M., Boughner, D. R., Ronald, J. A., Aldington, L., Rogers, K. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Development+of+aortic+valve+sclerosis+in+a+rabbit+model+of+atherosclerosis:+An+immunohistochemical+and+histological+study.">Development of aortic valve sclerosis in a rabbit model of atherosclerosis: An immunohistochemical and histological study.</a> Journal of Heart Valve Disease. 14 (3), 365-375 (2005).</li><li>Drolet, M. C., Couet, J., Arsenault, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Development+of+aortic+valve+sclerosis+or+stenosis+in+rabbits:+role+of+cholesterol+and+calcium.">Development of aortic valve sclerosis or stenosis in rabbits: role of cholesterol and calcium.</a> Journal of Heart Valve Disease. 17 (4), 381-387 (2008).</li><li>Sider, K. L., Blaser, M. C., Simmons, C. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Animal+models+of+calcific+aortic+valve+disease.">Animal models of calcific aortic valve disease.</a> International Journal of Inflammation. 2011, 364310 (2011).</li><li>Honda, S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+novel+mouse+model+of+aortic+valve+stenosis+induced+by+direct+wire+injury.">A novel mouse model of aortic valve stenosis induced by direct wire injury.</a> Arteriosclerosis, Thrombosis, and Vascular Biology. 34 (2), 270-278 (2014).</li><li>Niepmann, S. T., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Graded+murine+wire-induced+aortic+valve+stenosis+model+mimics+human+functional+and+morphological+disease+phenotype.">Graded murine wire-induced aortic valve stenosis model mimics human functional and morphological disease phenotype.</a> Clinical Research in Cardiology. 108 (8), 847-856 (2019).</li><li>Robbins, N., Thompson, A., Mann, A., Blomkalns, A. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Isolation+and+excision+of+murine+aorta;+A+versatile+technique+in+the+study+of+cardiovascular+disease.">Isolation and excision of murine aorta; A versatile technique in the study of cardiovascular disease.</a> Journal of Visualized Experiments. (93), e52172 (2014).</li><li>Wirrig, E. E., Gomez, M. V., Hinton, R. B., Yutzey, K. E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=COX2+inhibition+reduces+aortic+valve+calcification+in+vivo.">COX2 inhibition reduces aortic valve calcification in vivo.</a> Arteriosclerosis, Thrombosis, and Vascular Biology. 35 (4), 938-947 (2015).</li><li>Jung, S. H., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Spatiotemporal+dynamics+of+macrophage+heterogeneity+and+a+potential+function+of+Trem2(hi)+macrophages+in+infarcted+hearts.">Spatiotemporal dynamics of macrophage heterogeneity and a potential function of Trem2(hi) macrophages in infarcted hearts.</a> Nature Communications. 13 (1), 4580 (2022).</li><li>Freeman, R. V., Otto, C. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Spectrum+of+calcific+aortic+valve+disease:+Pathogenesis,+disease+progression,+and+treatment+strategies.">Spectrum of calcific aortic valve disease: Pathogenesis, disease progression, and treatment strategies.</a> Circulation. 111 (24), 3316-3326 (2005).</li><li>Lindman, B. R., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Calcific+aortic+stenosis.">Calcific aortic stenosis.</a> Nature Reviews Disease Primers. 2, 16006 (2016).</li><li>Cuniberti, L. A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Development+of+mild+aortic+valve+stenosis+in+a+rabbit+model+of+hypertension.">Development of mild aortic valve stenosis in a rabbit model of hypertension.</a> Journal of the American College of Cardiology. 47 (11), 2303-2309 (2006).</li><li>Marechaux, S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Identification+of+tissue+factor+in+experimental+aortic+valve+sclerosis.">Identification of tissue factor in experimental aortic valve sclerosis.</a> Cardiovascular Pathology. 18 (2), 67-76 (2009).</li><li>Hara, T., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Progression+of+calcific+aortic+valve+sclerosis+in+WHHLMI+rabbits.">Progression of calcific aortic valve sclerosis in WHHLMI rabbits.</a> Atherosclerosis. 273, 8-14 (2018).</li></ol>

动物 AVS 模型通常用于研究 AVS 的病理方面，包括 AVS 的发生和发展。该协议引入了一种新的兔 AVS 模型，该模型由主动脉瓣直接球囊损伤诱导。在这项研究中，主动脉瓣损伤模型显示出明显的瓣叶增厚和钙化。与膳食补充剂诱导的轻度AVS模型相比，直接球囊损伤模型中的主动脉瓣选择性损伤，导致瓣尖增厚和运动受限，以及小叶增厚和钙化。这些结果与AVS10,11<sup class="xref...

人们越来越认识到，由于无法获得与主动脉瓣狭窄 （AS） 进展相关的患病人主动脉瓣的可靠来源，使用适当的动物模型有助于更好地了解主动脉瓣狭窄 （AVS） 的潜在病理机制。在研究 AVS 的各种动物模型中，兔子是最常用的大型动物 AVS 模型之一，AVS 兔模型是通过补充胆固醇/维生素 D2 或基因操作诱导的 1,2,3,4。
尽管兔AVS模型为AVS的发展和进展提供了重要的见解，但正如我们的初步实验所看到的那样，一致且可重复地诱导AVS仍然具有挑战性。
除了饮食诱导和遗传易感的动物模型外，还通过小鼠的直接机械损伤建立了一种新的 AVS 模型 5,6。机械损伤模型成功诱导主动脉瓣狭窄，代表了野生型小鼠中简单且可重复的AVS模型。据我们所知，之前没有研究检查机械损伤对兔子模型中主动脉瓣的影响。因此，本研究提供了一种通过球囊直接损伤主动脉瓣诱导新西兰雄性大白兔AVS的新程序，可以准确模拟瓣膜性主动脉瓣狭窄的状况。该方案包括对准备、外科手术和术后护理的分步描述，这对于诱导可重复的AVS兔模型很有用。

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			<td>3-0 Silk suture</td>
			<td>AILEE</td>
			<td>SK312</td>
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			<td>4% paraformaldehyde(PFA)</td>
			<td>Intron</td>
			<td>IBS-BP031-2</td>
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			<td>Alizarin red Solution</td>
			<td>Millpore</td>
			<td>TMS-008-C</td>
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			<td>ASAHI SION BLUE&#160;</td>
			<td>ASAHI</td>
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			<td>Back Table Cover</td>
			<td>Yuhan kimberly</td>
			<td>80101-30</td>
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			<td>Balloon In-deflation Device</td>
			<td>Demax Medical</td>
			<td>DID30s</td>
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			<td>Bionet Veterinary monitor</td>
			<td>BIONET</td>
			<td>BM3 VET</td>
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			<td>C-Arm</td>
			<td>SIEMENS Healthcare GmbH</td>
			<td>Cios alpha</td>
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			<td>Certified Rabbit Diet</td>
			<td>Purina</td>
			<td>5322</td>
			<td>4.7% Hydrogenated Coconut Oil, 0.5% Cholesterol, &#38; 1% Molasse</td>
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			<td>Curadle Smart Incubator</td>
			<td>Autoelex</td>
			<td>CS-CV206</td>
			<td>Intensive Care Unit (ICU)</td>
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			<td>Ergocalciferol</td>
			<td>Sigma-aldrich&#160;</td>
			<td>E5750</td>
			<td>Vitamin D2</td>
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			<td>Fechtner conjunctiva forceps titanium</td>
			<td>WORLD PRECISSION Instrument</td>
			<td>WP1820</td>
			<td></td>
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			<td>Forceps</td>
			<td>HEBU</td>
			<td>HB203</td>
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			<td>Gentamicin</td>
			<td>Shin Poong</td>
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			<td>Glycopyrrolate&#160;</td>
			<td>SamChunDang</td>
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			<td>Greenflex NS</td>
			<td>DAI HAN PHARM</td>
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			<td>Normal saline 500 mL</td>
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			<td>Hematoxylin solution</td>
			<td>Sigma-aldrich&#160;</td>
			<td>HT1079-1 SET</td>
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			<td>Heparin</td>
			<td>JW pharmaceutical</td>
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			<td>25,000 U</td>
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			<td>Infusion set for single use</td>
			<td>SWOON MEDICAL</td>
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			<td>Iodine</td>
			<td>Green pharmaceutical</td>
			<td></td>
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			<td>Iodixanol</td>
			<td>GE Healthcare</td>
			<td>Visipaque</td>
			<td>Inflation solution (contrast agent)</td>
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			<td>IV catheter 22 G</td>
			<td>BD&#160;</td>
			<td>382423</td>
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			<td>IV catheter 24 G</td>
			<td>BD</td>
			<td>382412</td>
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			<td>Ketoprofen</td>
			<td>SamChunDang</td>
			<td></td>
			<td></td>
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			<td>Luer-Lok syringe 10 mL</td>
			<td>Becton Dickinson Medical</td>
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			<td>Luer-Lok syringe 3 mL</td>
			<td>Becton Dickinson Medical</td>
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			<td>Microscope</td>
			<td>OLYMPUS</td>
			<td>SZ61</td>
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			<td>Microtome</td>
			<td>ThermoFisher Scientific</td>
			<td>HM 325</td>
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			<td>MT stain kit</td>
			<td>Sigma-aldrich</td>
			<td>HT15-1kt</td>
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			<td>Needel holder</td>
			<td>Solco</td>
			<td>009-1304</td>
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			<td>Needle Holder with Lock and Suture</td>
			<td>JEUNGDO BIO &#38; PLANT</td>
			<td>H-1222-18</td>
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			<td>Paraffin</td>
			<td>LK LABKOREA</td>
			<td>H06-660-107</td>
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			<td>PBS</td>
			<td>Gibco</td>
			<td>10010-023</td>
			<td></td>
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			<td>Potassium chloride 40</td>
			<td>Daihan Pharm</td>
			<td></td>
			<td>KCl</td>
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			<td>Prelude Ideal Hydrophilic Sheath</td>
			<td>MERIT MEDICAL</td>
			<td>PID4F11018SS</td>
			<td>Sheath 4F</td>
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			<td>PTA Balloon Dilatation catheter</td>
			<td>Boston Scientific</td>
			<td>H749-3903280208-0</td>
			<td>Balloon catheter 8.0 mm</td>
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			<td>Rompun</td>
			<td>Elanco</td>
			<td></td>
			<td>Xylaxine</td>
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			<td>sterile Gauze</td>
			<td>DAE HAN Medical</td>
			<td></td>
			<td>10 cm x 20 cm&#160;</td>
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			<td>Surgical Gloves</td>
			<td>Ansell</td>
			<td>Ansell</td>
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			<td>Surgical Gown</td>
			<td>Yuhan kimberly</td>
			<td>90002-02</td>
			<td></td>
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			<td>Surgical Scissors</td>
			<td>Nopa, Germany</td>
			<td>AC020/16</td>
			<td></td>
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			<td>Surgical Tape</td>
			<td>3M micopore</td>
			<td>1530-1</td>
			<td></td>
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			<td>Syringe 1 mL</td>
			<td>Shin Chang Medical</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>Syringe 10 mL</td>
			<td>Shin Chang Medical</td>
			<td></td>
			<td></td>
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			<td>Tissue cassette</td>
			<td>Scilav korea</td>
			<td>Cas3003</td>
			<td></td>
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			<td>Transducer gel&#160;</td>
			<td>SUNGHEUNG</td>
			<td>SH102</td>
			<td></td>
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			<td>Tridol</td>
			<td>Yuhan Corp.</td>
			<td></td>
			<td>Tramadol HCl</td>
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		<tr>
			<td>Ultrasound system</td>
			<td>Philps</td>
			<td>Affiniti 50</td>
			<td></td>
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		<tr>
			<td>Von Kossa stain kit</td>
			<td>Abcam</td>
			<td>ab105689</td>
			<td></td>
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		<tr>
			<td>Zoletil 50</td>
			<td>Virbac korea</td>
			<td></td>
			<td>Tiletamine &#38; zolazepam</td>
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</table>

a rabbit aortic valve stenosis model induced by direct balloon injury

由于无法获得可靠的人主动脉瓣病源，动物模型正在成为了解主动脉瓣狭窄 （AVS） 潜在病理机制的重要工具。在各种动物模型中，AVS兔模型是大型动物研究中最常用的模型之一。然而，传统的AVS兔模型需要长期的膳食补充和基因操作才能诱导主动脉瓣的明显狭窄，限制了它们在实验研究中的使用。为了解决这些局限性，提出了一种新的AVS兔模型，其中狭窄是由主动脉瓣的直接球囊损伤引起的。本方案描述了一种在新西兰白兔（NZW）中诱导AVS的成功技术，包括准备，外科手术和术后护理的分步程序。这种简单且可重复的模型为研究AVS的发生和发展提供了一种有前途的方法，并为研究该疾病的潜在病理机制提供了有价值的工具。

主动脉瓣损伤诱导的兔AVS模型 为了诱导兔AVS模型，本研究使用体重3.5-4.0 kg的雄性NZW兔。根据步骤2（图2）中描述的外科手术，通过主动脉瓣损伤建立AVS模型，导致机械性主动脉瓣变性和钙化。对照组包括喂食富含 0.5% 胆固醇的饮食（高胆固醇，HC）和 50,000 U 维生素 D2 （VitD2） 的兔子，这被称为饮食诱导的 AVS 模型。
主动脉瓣的评估...

Watch this Scientific Journal Video about A Rabbit Aortic Valve Stenosis Model Induced by Direct Balloon Injury at JoVE.com

A Rabbit Aortic Valve Stenosis Model Induced by Direct Balloon Injury

需要适当的动物模型来了解主动脉瓣狭窄 （AVS） 的潜在病理机制并评估治疗干预的疗效。本方案描述了一种通过体内直接球囊损伤开发AVS兔模型的新程序。

球囊直接损伤诱导的兔主动脉瓣狭窄模型

Animal models are emerging as an important tool to understand the pathologic mechanisms underlying aortic valve stenosis (AVS) because of the lack of ...

由于无法获得患病的人类主动脉瓣的可靠来源，年度模型正在成为了解主动脉瓣狭窄潜在病理机制的重要工具。传统的主动脉瓣狭窄兔模型需要长期的膳食补充剂和基因操作来诱导主动脉瓣的显着狭窄，限制了它们在实验研究中的使用。该技术描述了一种通过对主动脉瓣的直接气球损伤来诱导兔子主动脉瓣狭窄的新程序，该程序可以准确地模拟主动脉瓣狭窄患者的状况。Eun-Hye Park、Jin-Moo Kim 和 Eunmi Lee 将演示该程序，他们是我所在监管机构的研究专家。通过将充满生理盐水和市售造影剂的一对一混合物的放气装置连接到球囊导管的鲁尔锁部分，开始准备扩张球囊导管组。用充气溶液填充球囊后，从球囊导管中排出空气。通过将 24 号静脉内或静脉导管插入麻醉兔的边缘耳静脉来开始手术程序。用每公斤100单位的肝素肝素盐水连接输液器。接下来，将兔子与多参数兽医监视器连接，以持续监测生命体征，例如氧饱和度信号或SpO2温度和血压。将兔子仰卧在配备C臂透视的手术台上后，使用动物毛发剪从腹侧颈部区域去除毛发。用碘对切口区域进行消毒，并用手术巾覆盖兔子。要定位兔子的心脏，请打开C型臂并选择透视模式进行心脏成像。调整兔子的位置，确保心脏位于成像场的中心。调整位置后，在颈部皮肤上做一个约3厘米的纵向切口，然后用手术剪刀切开筋膜和脂肪组织。然后小心地分开肌肉，直到暴露左颈总动脉（LCCA）约3至3.5厘米。在暴露的 LCCA 的顶部和末端用 3-0 丝线缝合 LCCA 以阻止血液流动。将 22 号 IV 导管插入 LCCA。然后通过静脉导管将一根 0.016 英寸的导丝引入左心室，确保导管尖端正确定位在 C 臂的成像区域中。接下来，抽出静脉导管，离开导丝，然后将 4 法式护套放在导丝上，放入 LCCA 以引入球囊导管。在C型臂透视引导下，小心地将8毫米球囊导管越过导丝插入主动脉瓣。将球囊导管尖端放置在主动脉瓣远端约一到两厘米处，并通过在六个大气压下用压力充气器吹扫充气溶液来给球囊充气。接下来，将球囊推进到左心室或左心室顶点，并将其拉回左心室出口。在给气球放气之前重复此过程五次。拔出球囊导管和导丝，然后慢慢从LCCA上取下护套，并立即将LCCA与缝合线结扎到主动脉瓣。用生理盐水清洁切口区域以去除血凝块。检查穿刺部位是否有动脉出血，然后用 3-0 不可吸收缝合线缝合肌肉和皮肤。完成后，用碘对伤口进行消毒。手术后，取下监测贴片和夹子，并将兔子放在重症监护保温箱中。球囊损伤八周后，将麻醉的兔子放在仰卧位的回声台上。剃除胸部区域后，将超声换能器凝胶涂抹在胸部。调整换能器以获得主动脉瓣的胸骨周围长轴视图和胸骨周围短轴视图。使用模式成像记录主动脉瓣的图像并保存图像以供以后分析。主动脉瓣结构变化评估显示，与对照或野生型兔相比，主动脉瓣损伤后8周，饲喂高胆固醇和维生素D2饮食的受伤兔的瓣尖增厚，运动受限，且饲喂高胆固醇和维生素D2饮食的兔无瓣膜损伤。在受伤八周后处死兔子并切除心脏，用于主动脉瓣的组织学分析。与高胆固醇和维生素 D2 饮食诱导组的对照组相比，用 Masson 三色染色的主动脉瓣显示受伤组主动脉瓣尖的厚度增加。茜素红染色和Von Kossa染色比较高胆固醇和维生素D2饮食诱导组瓣膜小叶中钙沉积的程度，表明钙沉积程度可忽略不计。相比之下，在球囊损伤组中观察到明显的钙化沉积物。本文演示了在兔子模型中诱导北极瓣膜狭窄的成功技术，并逐步解释了球囊对主动脉瓣的直接损伤。这种简单且可重复的模型为研究主动脉瓣狭窄的发生和发展提供了一种有前途的方法，并为研究主动脉瓣狭窄的潜在病理机制提供了有价值的工具。

Research

JoVE Journal

Medicine

Photothrombosis-induced Focal Ischemia as a Model of Spinal Cord Injury in Mice

Photothrombosis引起的局部脑缺血脊髓损伤的小鼠模型

Spinal cord injury (SCI) is a devastating clinical condition causing permanent changes in sensorimotor and autonomic functions of the spinal cord (SC) ...

科研

医学

A Rat Carotid Balloon Injury Model to Test Anti-vascular Remodeling Therapeutics

大鼠颈动脉球囊损伤模型试验抗血管重建治疗

The rat carotid balloon injury is a well-established surgical model that has been used to study arterial remodeling and vascular cell proliferation. It is ...

Reproducible Arterial Denudation Injury by Infrarenal Abdominal Aortic Clamping in a Murine Model

重复性的动脉剥脱损伤腹主动脉阻断在小鼠模型

Percutaneous vascular interventions uniformly result in arterial denudation injuries that subsequently lead to thrombosis and restenosis. These ...

The Rabbit Model of Accelerated Atherosclerosis: A Methodological Perspective of the Iliac Artery Balloon Injury

家兔加速动脉粥样硬化模型的研究--髂动脉球囊损伤的方法学观察

Acute coronary syndrome resulting from coronary occlusion following atherosclerotic plaque development and rupture is the leading cause of death in the ...

Full-root Aortic Valve Replacement by Stentless Aortic Xenografts in Patients with Small Aortic Roots

全根主动脉瓣置换主动脉瓣狭窄主动脉异种移植患者小主动脉根

In patients with small aortic roots who need an aortic valve replacement with biological valve substitutes, the implantation of the stented pericardial ...

Standardized Technique of Aortic Valve Re-implantation for Valve-sparing Aortic Root Replacement

主动脉瓣重置术治疗瓣膜备用主动脉根置换的规范化技术

Despite the obvious advantages of the preservation of a normal aortic valve during aortic root replacement, the complexity of valve sparing procedures ...

A Rabbit Model of Aqueous-Deficient Dry Eye Disease Induced by Concanavalin A Injection into the Lacrimal Glands: Application to Drug Efficacy Studies

由康卡纳林注射到乳原区引起的水性干眼病的兔子模型：药物功效研究的应用

Dry eye disease (DED), a multifactorial inflammatory disease of the ocular surface, affects 1 in 6 humans worldwide with staggering implications for ...

Inducing Acute Lung Injury in Mice by Direct Intratracheal Lipopolysaccharide Instillation

通过直接内切内脂多糖灌输诱导小鼠急性肺损伤

Airway administration of lipopolysaccharide (LPS) is a common way to study pulmonary inflammation and acute lung injury (ALI) in small animal models. ...

A Rat Carotid Artery Pressure-Controlled Segmental Balloon Injury with Periadventitial Therapeutic Application

大鼠胡萝卜动脉压力控制段气球损伤与腹腔ventit 治疗应用

Cardiovascular disease remains the leading cause of death and disability worldwide, in part due to atherosclerosis. Atherosclerotic plaque narrows the ...

Acute Kidney Injury Model Induced by Cisplatin in Adult Zebrafish

成人斑马鱼中西斯铂诱导的急性肾损伤模型

Cisplatin is commonly used as chemotherapy. Although it has positive effects in cancer-treated individuals, cisplatin can easily accumulate in the kidney ...