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Generation of Brown Fat-Specific Knockout Mice Using a Combined Cre-LoxP, CRISPR-Cas9, and Adeno-Associated Virus Single-Guide RNA System

DOI :

10.3791/65083-v

March 24th, 2023

March 24th, 2023

1,672 Views

1Section on Integrative Physiology and Metabolism, Research Division, Joslin Diabetes Center, Harvard Medical School

In this protocol, we describe the technical procedures to generate brown adipose tissue (BAT)-specific knockout mice leveraging a combined Cre-LoxP, CRISPR-Cas9, and adeno-associated virus (AAV) single-guide RNA (sgRNA) system. The described steps include the design of the sgRNAs, the preparation of the AAV-sgRNA particles, and the microinjection of AAV into the BAT lobes.

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Brown Fat specific Knockout Mice

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