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DOI :
10.3791/65126-v
•
8:02 min
March 3rd, 2023
Chapters
0:05
Introduction
0:43
Cell Culture and Harvesting
1:30
Microtubule Extraction
4:13
Cryo-Electron Microscopy (EM) Grid Preparation
6:16
Results: Analyzing Microtubule-Protein Complexes
7:34
Conclusion
ここでは、特定の微小管修飾酵素を欠くか含む可能性のある哺乳類細胞から内因性チューブリンを抽出し、特定の修飾のために富んだ微小管を得るためのプロトコルについて説明します。次に、抽出した微小管を精製した微小管結合タンパク質で修飾して、クライオ電子顕微鏡用のグリッドを準備する方法について説明します。
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